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Alternatives to animal-derived extracellular matrix hydrogels? An explorative study with HepaRG cells in animal-free hydrogels under static and dynamic culture conditions
ReferenceKatharina Stefanie Nitsche , Iris Müller , Sophie Malcomber , Paul Lawford Carmichael , Hans Bouwmeester Frontiers in Toxicology. 2025;7. doi: 10.3389/ftox.2025.1649393 New Approach Methodologies (NAMs) aim for an animal-free chemical risk assessment. …
Fetal bovine serum: how to leave it behind in the pursuit of more reliable science
ReferenceTilo Weber , Atena Malakpour-Permlid , Aline Chary , Vito D’Alessandro , Leah Haut , Sebastian Seufert , Esther Veronika Wenzel , James Hickman , Karen Bieback , Joachim Wiest , Wilhelm Gerhard Dirks , Sandra Coecke , Stina Oredsson Frontiers in …
Towards the standardization of human platelet lysate production and its comparison to fetal bovine serum for human hematopoietic cell culture: a scoping review
ReferenceShamili Immalaraju , Srishti Goyal , Rukmini Jonnalagadda Frontiers in Toxicology. 2025;7. doi: 10.3389/ftox.2025.1496231 Human hematopoietic cell culture (HCC) refers to the ex vivo growth of normal cells of the hematological system. These cells can be …
Optimizing Wnt activation in fetal calf serum (FCS)-free organoid expansion media
ReferenceEsther J. M. Liefting , Jeffrey J. Bajramovic Frontiers in Toxicology. 2025;7. doi: 10.3389/ftox.2025.1504469 Organoid technology can revolutionize biomedical research by increasing the translational value of experimental results while at the same time …
Use of serum-free media for peripheral blood mononuclear cell culture and the impact on T and B cell readouts
ReferenceStella Cochrane , Ouarda Saib , David Sheffield Frontiers in Toxicology. 2024;6. doi: 10.3389/ftox.2024.1462688 IntroductionAs part of a wider programme of work developing next-generation risk assessment approaches (NGRA) using non-animal methods (NAMs) …
Increasing sustainability and reproducibility of in vitro toxicology applications: serum-free cultivation of HepG2 cells
ReferenceLuisa Marie Pfeifer , Janike Sensbach , Frederic Pipp , Daniela Werkmann , Philip Hewitt Frontiers in Toxicology. 2024;6. doi: 10.3389/ftox.2024.1439031 Fetal Bovine Serum (FBS) is an important ingredient in cell culture media and the current standard for …
Advancing humanized 3D tumor modeling using an open access xeno-free medium
ReferenceAtena Malakpour-Permlid , Manuel Marcos Rodriguez , Kinga Zór , Anja Boisen , Stina Oredsson Frontiers in Toxicology. 2025;7. doi: 10.3389/ftox.2025.1529360 Despite limitations like poor mimicry of the human cell microenvironment, contamination risks, and …
Gastruloids enable modeling of the earliest stages of human cardiac and hepatic vascularization
ReferenceOscar J. Abilez , Huaxiao Yang , Yuan Guan , Mengcheng Shen , Zehra Yildirim , Yan Zhuge , Ravichandra Venkateshappa , Shane R. Zhao , Angello H. Gomez , Marcel El-Mokahal , Logan Dunkenberger , Yoshikazu Ono , Masafumi Shibata , Peter N. Nwokoye , Lei …
Cardiometabolic disease and cardio-oncology: Insights from iPSC models and tissue engineering
ReferenceAna Kojic , Javid Moslehi , Bonnie Ky , Joseph C. Wu Cell Reports Medicine. 2025;6(9):102261. doi: 10.1016/j.xcrm.2025.102261 Heart disease and cancer share common risk factors, genetic predispositions, and metabolic and inflammatory components. Metabolic …
Should culture media for microphysiological systems be nutritionally physiological?
ReferenceAlastair G. Stewart , Avanka Gunatilaka , Bryan Gao Nature Reviews Bioengineering. 2025;3(5):357-358. doi: 10.1038/s44222-025-00290-w The incipient use of microphysiological systems (MPSs) in the drug regulatory pathway is based on equivalent or superior …