Media and materials

Manuela Cassotta, Joanna Julia Bartnicka, Francesca Pistollato, et al. A worldwide survey on the use of animal-derived materials and reagents in scientific experimentation. Engineering in Life Sciences. 2022;22(9):564-583. doi:10.1002/elsc.202100167

Ornella Urzì, Roger Olofsson Bagge, Rossella Crescitelli. The dark side of foetal bovine serum in extracellular vesicle studies. Journal of Extracellular Vesicles. 2022;11(10):e12271. doi:10.1002/jev2.12271

Alison Gray, Andrew R. M. Bradbury, Achim Knappik, Andreas Plückthun, Carl A. K. Borrebaeck, Stefan Dübel. Animal-free alternatives and the antibody iceberg. Nature Biotechnology. 2020;38(11):1234-1239. doi:10.1038/s41587-020-0687-9

Katherine Groff, David Allen, Warren Casey, Amy Clippinger. Increasing the use of animal-free recombinant antibodies. ALTEX. 2020;37(2):309-311. doi:10.14573/altex.2001071

Joint Research Centre (European Commission), J. Barroso, M. Halder, M. Whelan. EURL ECVAM Recommendation on Non-Animal-Derived Antibodies. Publications Office of the European Union; 2020. https://data.europa.eu/doi/10.2760/80554.

Mathias Uhlen, Anita Bandrowski, Steven Carr, et al. A proposal for validation of antibodies. Nature Methods. 2016;13(10):823-827. doi:10.1038/nmeth.3995

A. C. Gray, S. S. Sidhu, P. C. Chandrasekera, C. F. M. Hendriksen, C. a. K. Borrebaeck. Animal-Friendly Affinity Reagents: Replacing the Needless in the Haystack. Trends in Biotechnology. 2016;34(12):960-969. doi:10.1016/j.tibtech.2016.05.017

Zhiyun Wei, Arsen O. Batagov, David R. F. Carter, Anna M. Krichevsky. Fetal Bovine Serum RNA Interferes with the Cell Culture derived Extracellular RNA. Scientific Reports. 2016;6(1):31175. doi:10.1038/srep31175

Monya Baker. Reproducibility crisis: Blame it on the antibodies. Nature. 2015;521(7552):274-276. doi:10.1038/521274a

Lida A. Beninson, Monika Fleshner. Exosomes in fetal bovine serum dampen primary macrophage IL-1β response to lipopolysaccharide (LPS) challenge. Immunology Letters. 2015;163(2):187-192. doi:10.1016/j.imlet.2014.10.019