Media and materials

Luisa Marie Pfeifer, Janike Sensbach, Frederic Pipp, Daniela Werkmann, Philip Hewitt. Increasing sustainability and reproducibility of in vitro toxicology applications: serum-free cultivation of HepG2 cells. Frontiers in Toxicology. 2024;6. doi:10.3389/ftox.2024.1439031

Tilo Weber, Jeffrey Bajramovic, Stina Oredsson. Preparation of a universally usable, animal product free, defined medium for 2D and 3D culturing of normal and cancer cells. MethodsX. 2024;12:102592. doi:10.1016/j.mex.2024.102592

Chad P. Nelson, Paul Brown, Suzanne Fitzpatrick, et al. Advancing alternative methods to reduce animal testing. Science. 2024;386(6723):724-726. doi:10.1126/science.adg6228

Ornella Urzì, Roger Olofsson Bagge, Rossella Crescitelli. The dark side of foetal bovine serum in extracellular vesicle studies. Journal of Extracellular Vesicles. 2022;11(10):e12271. doi:10.1002/jev2.12271

Manuela Cassotta, Joanna Julia Bartnicka, Francesca Pistollato, et al. A worldwide survey on the use of animal-derived materials and reagents in scientific experimentation. Engineering in Life Sciences. 2022;22(9):564-583. doi:10.1002/elsc.202100167

Joint Research Centre (European Commission), J. Barroso, M. Halder, M. Whelan. EURL ECVAM Recommendation on Non-Animal-Derived Antibodies. Publications Office of the European Union; 2020. https://data.europa.eu/doi/10.2760/80554.

Alison Gray, Andrew R. M. Bradbury, Achim Knappik, Andreas Plückthun, Carl A. K. Borrebaeck, Stefan Dübel. Animal-free alternatives and the antibody iceberg. Nature Biotechnology. 2020;38(11):1234-1239. doi:10.1038/s41587-020-0687-9

Katherine Groff, David Allen, Warren Casey, Amy Clippinger. Increasing the use of animal-free recombinant antibodies. ALTEX. 2020;37(2):309-311. doi:10.14573/altex.2001071

Zhiyun Wei, Arsen O. Batagov, David R. F. Carter, Anna M. Krichevsky. Fetal Bovine Serum RNA Interferes with the Cell Culture derived Extracellular RNA. Scientific Reports. 2016;6(1):31175. doi:10.1038/srep31175

Mathias Uhlen, Anita Bandrowski, Steven Carr, et al. A proposal for validation of antibodies. Nature Methods. 2016;13(10):823-827. doi:10.1038/nmeth.3995