1.
Esther J. M. Liefting, Jeffrey J. Bajramovic. Optimizing Wnt activation in fetal calf serum (FCS)-free organoid expansion media. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1504469
Media and materials
1.
Shamili Immalaraju, Srishti Goyal, Rukmini Jonnalagadda. Towards the standardization of human platelet lysate production and its comparison to fetal bovine serum for human hematopoietic cell culture: a scoping review. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1496231
1.
Tilo Weber, Atena Malakpour-Permlid, Aline Chary, et al. Fetal bovine serum: how to leave it behind in the pursuit of more reliable science. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1612903
1.
Katharina Stefanie Nitsche, Iris Müller, Sophie Malcomber, Paul Lawford Carmichael, Hans Bouwmeester. Alternatives to animal-derived extracellular matrix hydrogels? An explorative study with HepaRG cells in animal-free hydrogels under static and dynamic culture conditions. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1649393
1.
Alexandra Nessar, Viola Röhrs, Mathias Ziersch, et al. Promoting ethical and reproducible cell culture: implementing animal-free alternatives to teaching in molecular and cell biology. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1670513
1.
Valeria Di Leonardo, Julia Brenmoehl, Heike Wanka, Bianka Grunow. Proliferation and metabolic activity of the Atlantic sturgeon cell line AOXlar7y under short-term serum-reduced conditions, and the effect of stimulation with growth factors and cytokines. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1636776
1.
Stina Oredsson, Atena Malakpour-Permlid, Johan Zhu, Tilo Weber. Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures. STAR Protocols. 2025;6(2):103781. doi:10.1016/j.xpro.2025.103781
1.
Wim B. M. de Lau, Joost J. A. P. M. Wijnakker, Gijs J. F. van Son, et al. A single-chain derivative of an integrin-activating antibody potentiates organoid growth in Matrigel and collagen hydrogels. Nature Biotechnology. 2025:1-11. doi:10.1038/s41587-025-02874-8
1.
Lisa Wolff, Sven Hendrix. Rethinking Matrigel: The Complex Journey to Matrix Alternatives in Organoid Culture. Advanced Science. 2025;12(47):e08734. doi:10.1002/advs.202508734
1.
Stella Cochrane, Ouarda Saib, David Sheffield. Use of serum-free media for peripheral blood mononuclear cell culture and the impact on T and B cell readouts. Frontiers in Toxicology. 2024;6. doi:10.3389/ftox.2024.1462688