Media and materials

Stina Oredsson, Atena Malakpour-Permlid, Johan Zhu, Tilo Weber. Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures. STAR Protocols. 2025;6(2):103781. doi:10.1016/j.xpro.2025.103781

Alastair G. Stewart, Avanka Gunatilaka, Bryan Gao. Should culture media for microphysiological systems be nutritionally physiological? Nature Reviews Bioengineering. 2025;3(5):357-358. doi:10.1038/s44222-025-00290-w

Atena Malakpour-Permlid, Manuel Marcos Rodriguez, Kinga Zór, Anja Boisen, Stina Oredsson. Advancing humanized 3D tumor modeling using an open access xeno-free medium. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1529360

Esther J. M. Liefting, Jeffrey J. Bajramovic. Optimizing Wnt activation in fetal calf serum (FCS)-free organoid expansion media. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1504469

Shamili Immalaraju, Srishti Goyal, Rukmini Jonnalagadda. Towards the standardization of human platelet lysate production and its comparison to fetal bovine serum for human hematopoietic cell culture: a scoping review. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1496231

Tilo Weber, Atena Malakpour-Permlid, Aline Chary, et al. Fetal bovine serum: how to leave it behind in the pursuit of more reliable science. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1612903

Katharina Stefanie Nitsche, Iris Müller, Sophie Malcomber, Paul Lawford Carmichael, Hans Bouwmeester. Alternatives to animal-derived extracellular matrix hydrogels? An explorative study with HepaRG cells in animal-free hydrogels under static and dynamic culture conditions. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1649393

Alexandra Nessar, Viola Röhrs, Mathias Ziersch, et al. Promoting ethical and reproducible cell culture: implementing animal-free alternatives to teaching in molecular and cell biology. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1670513

Chad P. Nelson, Paul Brown, Suzanne Fitzpatrick, et al. Advancing alternative methods to reduce animal testing. Science. 2024;386(6723):724-726. doi:10.1126/science.adg6228

Tilo Weber, Jeffrey Bajramovic, Stina Oredsson. Preparation of a universally usable, animal product free, defined medium for 2D and 3D culturing of normal and cancer cells. MethodsX. 2024;12:102592. doi:10.1016/j.mex.2024.102592