Media and materials

Alexandra Nessar, Viola Röhrs, Mathias Ziersch, et al. Promoting ethical and reproducible cell culture: implementing animal-free alternatives to teaching in molecular and cell biology. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1670513

Wim B. M. de Lau, Joost J. A. P. M. Wijnakker, Gijs J. F. van Son, et al. A single-chain derivative of an integrin-activating antibody potentiates organoid growth in Matrigel and collagen hydrogels. Nature Biotechnology. 2025:1-11. doi:10.1038/s41587-025-02874-8

Valeria Di Leonardo, Julia Brenmoehl, Heike Wanka, Bianka Grunow. Proliferation and metabolic activity of the Atlantic sturgeon cell line AOXlar7y under short-term serum-reduced conditions, and the effect of stimulation with growth factors and cytokines. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1636776

Niraj Chaudhary, Luis G. Villa-Diaz. Advancements in the in vitro culture of human pluripotent stem cells: progress, challenges, and future directions: comprehensive review. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1667573

Alastair G. Stewart, Avanka Gunatilaka, Bryan Gao. Should culture media for microphysiological systems be nutritionally physiological? Nature Reviews Bioengineering. 2025;3(5):357-358. doi:10.1038/s44222-025-00290-w

Atena Malakpour-Permlid, Manuel Marcos Rodriguez, Kinga Zór, Anja Boisen, Stina Oredsson. Advancing humanized 3D tumor modeling using an open access xeno-free medium. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1529360

Esther J. M. Liefting, Jeffrey J. Bajramovic. Optimizing Wnt activation in fetal calf serum (FCS)-free organoid expansion media. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1504469

Shamili Immalaraju, Srishti Goyal, Rukmini Jonnalagadda. Towards the standardization of human platelet lysate production and its comparison to fetal bovine serum for human hematopoietic cell culture: a scoping review. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1496231

Tilo Weber, Atena Malakpour-Permlid, Aline Chary, et al. Fetal bovine serum: how to leave it behind in the pursuit of more reliable science. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1612903

Katharina Stefanie Nitsche, Iris Müller, Sophie Malcomber, Paul Lawford Carmichael, Hans Bouwmeester. Alternatives to animal-derived extracellular matrix hydrogels? An explorative study with HepaRG cells in animal-free hydrogels under static and dynamic culture conditions. Frontiers in Toxicology. 2025;7. doi:10.3389/ftox.2025.1649393