%0 Journal Article %K chemical defined cell culture systems %K developmental neurotoxicity assays %K human cell-based testing %K Regulatory toxicology %K xeno-free cell culture %B Frontiers in Toxicology %D 2026 %G English %R 10.3389/ftox.2026.1800157 %T Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays %U https://www.frontiersin.org/journals/toxicology/articles/10.3389/ftox.2026.1800157/full %V 8 %8 2026-05-12 %! Challenges and solutions in transitioning to animal-free standards