%0 Journal Article %K feeder-free culture %K xeno-free substrates %B Frontiers in Toxicology %D 2025 %G English %R 10.3389/ftox.2025.1667573 %T Advancements in the in vitro culture of human pluripotent stem cells: progress, challenges, and future directions: comprehensive review %U https://www.frontiersin.org/journals/toxicology/articles/10.3389/ftox.2025.1667573/full %V 7 %8 2025-12-02 %! Advancements in the in vitro culture of human pluripotent stem cells