TY - JOUR KW - Biomaterials – cells KW - Biomedical Engineering KW - Mechanical engineering KW - Tissue engineering AU - Chao-Fan He AU - Tian-Hong Qiao AU - Guang-Hao Wang AU - Yuan Sun AU - Yong He AB - Projection-based 3D printing is a vat polymerization printing method that works by generating bitmaps as dynamic masks to project onto a photosensitive material surface for layer-by-layer curing. Projection-based 3D printing has the highest resolution/time for manufacturing ratio among all 3D printing technologies; however, projection-based 3D bioprinting, which uses bioinks as printing materials that contain cells and/or biomolecules, suffers from low printing resolution, with a substantial gap between the theoretical and the actual resolution. In this Review, we summarize the steps and challenges to achieve high-resolution projection-based 3D bioprinting and provide pragmatic optimization strategies for tissue engineering and regenerative medicine applications. BT - Nature Reviews Bioengineering DA - 2025-02 DO - 10.1038/s44222-024-00218-w IS - 2 LA - en N2 - Projection-based 3D printing is a vat polymerization printing method that works by generating bitmaps as dynamic masks to project onto a photosensitive material surface for layer-by-layer curing. Projection-based 3D printing has the highest resolution/time for manufacturing ratio among all 3D printing technologies; however, projection-based 3D bioprinting, which uses bioinks as printing materials that contain cells and/or biomolecules, suffers from low printing resolution, with a substantial gap between the theoretical and the actual resolution. In this Review, we summarize the steps and challenges to achieve high-resolution projection-based 3D bioprinting and provide pragmatic optimization strategies for tissue engineering and regenerative medicine applications. PY - 2025 SP - 143 EP - 158 T2 - Nature Reviews Bioengineering TI - High-resolution projection-based 3D bioprinting UR - https://www.nature.com/articles/s44222-024-00218-w VL - 3 Y2 - 2026-08-12 SN - 2731-6092 ER -