TY - JOUR
KW - Cell biology
KW - Cellular imaging
KW - Image processing
KW - Stem-cell differentiation
AU - Caroline Hookway
AU - Antoine Borensztejn
AU - Leigh K. Harris
AU - Tiffany Barszczewski
AU - Sara Carlson
AU - Gokhan Dalgin
AU - Suraj Mishra
AU - Ellen M. Adams
AU - Julie C. Dixon
AU - Renske J. Dupar
AU - Jacqueline H. Edmonds
AU - Erik A. Ehlers
AU - Alexandra J. Ferrante
AU - Margaret A. Fuqua
AU - Clare R. Gamlin
AU - Philip Garrison
AU - Janani Gopalan
AU - Benjamin W. Gregor
AU - Maxwell J. Hedayati
AU - Victoria L. Hurless
AU - Kyle N. Klein
AU - Chantelle L. Leveille
AU - Sean L. Meharry
AU - Ricardo Mercado
AU - Haley S. Morris
AU - Gouthamrajan Nadarajan
AU - Nivedita Nivedita
AU - Sandra A. Oluoch
AU - Serge E. Parent
AU - Amber Phan
AU - Brock Roberts
AU - Ashwin Samudre
AU - Emmanuel E. Sanchez
AU - M. Filip Sluzewski
AU - Lev S. Snyder
AU - Derek J. Thirstrup
AU - Hannah F. Thorp
AU - John Paul Thottam
AU - Julia R. Torvi
AU - Gaea Turman
AU - Matheus P. Viana
AU - Lyndsay Wilhelm
AU - Chamari S. Wijesooriya
AU - Jie Yao
AU - Julie A. Theriot
AU - Ruwanthi N. Gunawardane
AU - Susanne M. Rafelski
AB - The epithelial-to-mesenchymal transition (EMT) is a widely studied cell state change, yet differences in model design and measurement approaches limit comparison across studies. Addressing this challenge requires experimental model systems and analysis frameworks that support standardization across contexts. Here, we show that human induced pluripotent stem (hiPS) cells in defined cell culture geometries, two-dimensional colonies and three-dimensional lumenoids, enable multimodal measurements of EMT dynamics within a single experimental platform. Using fixed-cell and live-cell image-based assays, we quantify changes in cell migration, EMT-related molecular markers, cell–cell junction organization and interactions with the basement membrane, a specialized form of the extracellular matrix, during EMT induced in hiPS cells. We identify cell culture geometry-dependent differences in the timing of migration onset and show that basement membrane integrity can be quantitatively linked to these differences. Together, these results establish an imaging-based framework for analysis of cell state transitions and provide accessible datasets and tools.
BT - Nature Methods
DA - 2026-06-15
DO - 10.1038/s41592-026-03096-9
LA - en
N2 - The epithelial-to-mesenchymal transition (EMT) is a widely studied cell state change, yet differences in model design and measurement approaches limit comparison across studies. Addressing this challenge requires experimental model systems and analysis frameworks that support standardization across contexts. Here, we show that human induced pluripotent stem (hiPS) cells in defined cell culture geometries, two-dimensional colonies and three-dimensional lumenoids, enable multimodal measurements of EMT dynamics within a single experimental platform. Using fixed-cell and live-cell image-based assays, we quantify changes in cell migration, EMT-related molecular markers, cell–cell junction organization and interactions with the basement membrane, a specialized form of the extracellular matrix, during EMT induced in hiPS cells. We identify cell culture geometry-dependent differences in the timing of migration onset and show that basement membrane integrity can be quantitatively linked to these differences. Together, these results establish an imaging-based framework for analysis of cell state transitions and provide accessible datasets and tools.
PY - 2026
SP - 1
EP - 13
T2 - Nature Methods
TI - A human induced pluripotent stem cell model for the holistic study of epithelial-to-mesenchymal transitions
UR - https://www.nature.com/articles/s41592-026-03096-9
Y2 - 2026-06-22
SN - 1548-7105
ER -