TY - JOUR
KW - Apical secretion
KW - Endometrial organoids
KW - Infertile organoids
KW - Intra-organoid fluid
KW - blastocyst attachment
AU - Wei Zhou
AU - Siena Barton
AU - Jinwei Cui
AU - Leilani L. Santos
AU - Guannan Yang
AU - Catharyn Stern
AU - Violet Kieu
AU - Wan Tinn Teh
AU - Catarina Ang
AU - Tarana Lucky
AU - Joseph Sgroi
AU - Louie Ye
AU - Evdokia Dimitriadis
AB - Embryo implantation failure leads to infertility. As an important approach to regulate implantation, endometrial epithelial cells produce and secrete factors apically into the uterine cavity in the receptive phase to prepare the initial blastocyst adhesion and implantation. We established organoids using endometrial biopsies from women with normal fertility and primary infertility and treated them with hormones to model the receptive phase endometrial epithelium. Our data show that infertile organoids were dysregulated in their response to estrogen and progesterone treatment. Proteomic analysis of organoid apical secretions identified 150 dysregulated proteins between fertile and infertile groups (>1.5-fold change). Trophoblast progenitor spheroids (blastocyst surrogates) treated with infertile organoid apical secretions significantly compromised their adhesion to organoid epithelial cell monolayers compared to fertile group (P < 0.0001). Overall, this study paves the way to determine the molecular mechanisms by which endometrial epithelial apical released factors regulate blastocyst initial attachment and implantation.
BT - Frontiers in Endocrinology
DA - 2022-12-14
DO - 10.3389/fendo.2022.1067648
LA - English
N2 - Embryo implantation failure leads to infertility. As an important approach to regulate implantation, endometrial epithelial cells produce and secrete factors apically into the uterine cavity in the receptive phase to prepare the initial blastocyst adhesion and implantation. We established organoids using endometrial biopsies from women with normal fertility and primary infertility and treated them with hormones to model the receptive phase endometrial epithelium. Our data show that infertile organoids were dysregulated in their response to estrogen and progesterone treatment. Proteomic analysis of organoid apical secretions identified 150 dysregulated proteins between fertile and infertile groups (>1.5-fold change). Trophoblast progenitor spheroids (blastocyst surrogates) treated with infertile organoid apical secretions significantly compromised their adhesion to organoid epithelial cell monolayers compared to fertile group (P < 0.0001). Overall, this study paves the way to determine the molecular mechanisms by which endometrial epithelial apical released factors regulate blastocyst initial attachment and implantation.
PY - 2022
T2 - Frontiers in Endocrinology
TI - Infertile human endometrial organoid apical protein secretions are dysregulated and impair trophoblast progenitor cell adhesion
UR - https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2022.1067648/full
VL - 13
Y2 - 2026-06-03
SN - 1664-2392
ER -