TY - JOUR
KW - Cell biology
KW - Cell-based assays
KW - Molecular biology
KW - organoids
KW - Signal Transduction
KW - Single cell
KW - Stem cells
AU - Kari-Pekka Skarp
AU - Bahar Yetkin-Arik
AU - Suze A. Jansen
AU - Caroline A. Lindemans
AU - Magdalena J. Lorenowicz
AB - Mesenchymal stem/stromal cells (MSCs) are known for their regenerative properties. This protocol describes a co-culture system for investigating molecular interactions between MSCs and intestinal epithelial organoids following injury. We outline steps for assessing the immediate effects of MSCs on organoid growth and survival, as well as a model for evaluating longer term responses. The workflow is adaptable and can be readily modified to examine MSC interactions with additional cell types or in different injury contexts. For complete information on the use and execution of this protocol, please refer to Yetkin-Arik et al.
BT - STAR protocols
DA - 2025-12-24
DO - 10.1016/j.xpro.2025.104292
IS - 1
LA - eng
N2 - Mesenchymal stem/stromal cells (MSCs) are known for their regenerative properties. This protocol describes a co-culture system for investigating molecular interactions between MSCs and intestinal epithelial organoids following injury. We outline steps for assessing the immediate effects of MSCs on organoid growth and survival, as well as a model for evaluating longer term responses. The workflow is adaptable and can be readily modified to examine MSC interactions with additional cell types or in different injury contexts. For complete information on the use and execution of this protocol, please refer to Yetkin-Arik et al.
PY - 2025
EP - 104292
T2 - STAR protocols
TI - Protocol for modeling the repair of intestinal damage by co-culturing mesenchymal stromal/stem cells and intestinal organoids
VL - 7
SN - 2666-1667
ER -