TY - JOUR
KW - Capillary Malformation
KW - Endothelial Cells
KW - human induced pluripotent stem cells
KW - Smooth Muscle Cells
KW - Vascular Organoid
AU - Vi Nguyen
AU - Anna Harper
AU - Mackenzie Azuero
AU - Isabella Castellanos
AU - Siwuxie He
AU - Marcelo L. Hochman
AU - Camilla F. Wenceslau
AU - Dong-bao Chen
AU - Anil G. Jegga
AU - Yunguan Wang
AU - Daping Fan
AU - J. Stuart Nelson
AU - Wenbin Tan
AB - Capillary malformation (CM) is a congenital vascular anomaly that affects the skin, mucosa, eye, and brain. A major obstacle to mechanistic and drug screening studies for CM has been the lack of preclinical models. In this study, we established vascular organoids (VOs) generated through the self-assembly of vascular lineages of endothelial cells and smooth muscle cells differentiated from CM-induced pluripotent stem cells (iPSC). Within these VOs induced endothelial cells and smooth muscle cells organized into juxtapositions to form vascular branches. CM patient iPSC-derived VOs showed a higher density of endothelial and smooth muscle cell populations and greater vascular branch lengths as compared with VOs derived from iPSCs generated from healthy skin biopsies. Overall, this study represents the first disease-relevant VO model of CM, providing a valuable platform for future mechanistic studies and drug screening.
BT - Stem Cell Reviews and Reports
DA - 2025-09-25
DO - 10.1007/s12015-025-10984-8
LA - en
N2 - Capillary malformation (CM) is a congenital vascular anomaly that affects the skin, mucosa, eye, and brain. A major obstacle to mechanistic and drug screening studies for CM has been the lack of preclinical models. In this study, we established vascular organoids (VOs) generated through the self-assembly of vascular lineages of endothelial cells and smooth muscle cells differentiated from CM-induced pluripotent stem cells (iPSC). Within these VOs induced endothelial cells and smooth muscle cells organized into juxtapositions to form vascular branches. CM patient iPSC-derived VOs showed a higher density of endothelial and smooth muscle cell populations and greater vascular branch lengths as compared with VOs derived from iPSCs generated from healthy skin biopsies. Overall, this study represents the first disease-relevant VO model of CM, providing a valuable platform for future mechanistic studies and drug screening.
PY - 2025
T2 - Stem Cell Reviews and Reports
TI - Vascular Organoids Derived from Capillary malformation-induced Pluripotent Stem Cells Exhibit Disease-Relevant Phenotypes
UR - https://doi.org/10.1007/s12015-025-10984-8
Y2 - 2025-12-30
SN - 2629-3277
ER -