TY - JOUR
KW - Biotechnology
KW - medical research
AU - Wim B. M. de Lau
AU - Joost J. A. P. M. Wijnakker
AU - Gijs J. F. van Son
AU - Daniel Krueger
AU - Daisong Wang
AU - Matthijs S. Abendroth
AU - Robin Schreurs
AU - Claudia Y. Janda
AU - Fenna L. H. van Rijt
AU - Benjamin J. P. Chalopin
AU - Emma Bokobza
AU - Johan H. van Es
AU - Timothy A. Springer
AU - Arnoud Sonnenberg
AU - Hans Clevers
AB - Current methods of culturing human epithelial organoids from adult stem cells may not be compatible with clinical applications as they rely on xenogeneic, chemically undefined or non-standardized components such as the basement membrane extract Matrigel. Matrigel provides a source of extracellular matrix molecules, including laminins and collagen IV, which interact with β1 integrins expressed on organoid cells. Here we describe a single-chain (sc) version of antibody TS2/16 that allosterically activates integrin β1 function in organoids. The addition of monomeric scTS2/16 to organoid medium results in up to a fivefold increase in the yield of all gastrointestinal organoids grown in Matrigel. Moreover, scTS2/16 supports a six- to sevenfold increase in the yield of these organoids when cultured in collagen I hydrogels, both in 3D and 2D. Collagen I is well defined, available in clinical-grade formulations and, when combined with scTS2/16, may support the clinical application of epithelial organoids derived from gastrointestinal tissues and other epithelial sources.
BT - Nature Biotechnology
DA - 2025-10-28
DO - 10.1038/s41587-025-02874-8
LA - en
N2 - Current methods of culturing human epithelial organoids from adult stem cells may not be compatible with clinical applications as they rely on xenogeneic, chemically undefined or non-standardized components such as the basement membrane extract Matrigel. Matrigel provides a source of extracellular matrix molecules, including laminins and collagen IV, which interact with β1 integrins expressed on organoid cells. Here we describe a single-chain (sc) version of antibody TS2/16 that allosterically activates integrin β1 function in organoids. The addition of monomeric scTS2/16 to organoid medium results in up to a fivefold increase in the yield of all gastrointestinal organoids grown in Matrigel. Moreover, scTS2/16 supports a six- to sevenfold increase in the yield of these organoids when cultured in collagen I hydrogels, both in 3D and 2D. Collagen I is well defined, available in clinical-grade formulations and, when combined with scTS2/16, may support the clinical application of epithelial organoids derived from gastrointestinal tissues and other epithelial sources.
PY - 2025
SP - 1
EP - 11
T2 - Nature Biotechnology
TI - A single-chain derivative of an integrin-activating antibody potentiates organoid growth in Matrigel and collagen hydrogels
UR - https://www.nature.com/articles/s41587-025-02874-8
Y2 - 2025-12-02
SN - 1546-1696
ER -