TY - JOUR
KW - Developmental neurogenesis
KW - Extracellular recording
KW - induced pluripotent stem cells
KW - Neurophysiology
AU - Tjitse van der Molen
AU - Alex Spaeth
AU - Mattia Chini
AU - Sebastian Hernandez
AU - Gregory A. Kaurala
AU - Hunter E. Schweiger
AU - Cole Duncan
AU - Sawyer McKenna
AU - Jinghui Geng
AU - Max Lim
AU - Julian Bartram
AU - Tobias Gänswein
AU - Aditya Dendukuri
AU - Zongren Zhang
AU - Jesus Gonzalez-Ferrer
AU - Kiran Bhaskaran-Nair
AU - Aidan L. Morson
AU - Cole R. K. Harder
AU - Linda R. Petzold
AU - Dowlette-Mary Alam El Din
AU - Jason Laird
AU - Maren Schenke
AU - Lena Smirnova
AU - Bradley M. Colquitt
AU - Mohammed A. Mostajo-Radji
AU - Paul K. Hansma
AU - Mircea Teodorescu
AU - Andreas Hierlemann
AU - Keith B. Hengen
AU - Ileana L. Hanganu-Opatz
AU - Kenneth S. Kosik
AU - Tal Sharf
AB - Neuronal firing sequences are thought to be the building blocks of information and broadcasting within the brain. Yet, it remains unclear when these sequences emerge during neurodevelopment. Here we demonstrate that structured firing sequences appear in spontaneous activity of human and murine brain organoids, both unguided and forebrain identity directed, as well as ex vivo neonatal murine cortical slices. We observed temporally rigid and flexible firing patterns in human and murine brain organoids and early postnatal murine somatosensory cortex, but not in dissociated primary cortical cultures. These results suggest that temporal sequences do not arise in an experience-dependent manner, but are rather constrained by a preconfigured architecture established during neurodevelopment. By demonstrating the developmental recapitulation of neural firing patterns, these findings highlight the potential of brain organoids as a model for neuronal circuit assembly.
BT - Nature Neuroscience
DA - 2025-11-24
DO - 10.1038/s41593-025-02111-0
LA - en
N2 - Neuronal firing sequences are thought to be the building blocks of information and broadcasting within the brain. Yet, it remains unclear when these sequences emerge during neurodevelopment. Here we demonstrate that structured firing sequences appear in spontaneous activity of human and murine brain organoids, both unguided and forebrain identity directed, as well as ex vivo neonatal murine cortical slices. We observed temporally rigid and flexible firing patterns in human and murine brain organoids and early postnatal murine somatosensory cortex, but not in dissociated primary cortical cultures. These results suggest that temporal sequences do not arise in an experience-dependent manner, but are rather constrained by a preconfigured architecture established during neurodevelopment. By demonstrating the developmental recapitulation of neural firing patterns, these findings highlight the potential of brain organoids as a model for neuronal circuit assembly.
PY - 2025
SP - 1
EP - 13
T2 - Nature Neuroscience
TI - Preconfigured neuronal firing sequences in human brain organoids
UR - https://www.nature.com/articles/s41593-025-02111-0
Y2 - 2025-12-01
SN - 1546-1726
ER -