TY - JOUR
KW - 3D bioprinting
KW - cancer metastasis
KW - direct ink writing
KW - organ model
KW - perfusion
KW - sacrificial-free
KW - vascularization
AU - Dongwei Wu
AU - Shumin Pang
AU - Johanna Berg
AU - Yikun Mei
AU - Ahmed S. M. Ali
AU - Viola Röhrs
AU - Beatrice Tolksdorf
AU - Judith Hagenbuchner
AU - Michael J. Ausserlechner
AU - Hedwig E. Deubzer
AU - Aleksander Gurlo
AU - Jens Kurreck
AB - 3D bioprinting enables the fabrication of human organ models that can be used for various fields of biomedical research, including oncology and infection biology. An important challenge, however, remains the generation of vascularized, perfusable 3D models that closely simulate natural physiology. Here, a novel direct ink writing (DIW) approach is described that can produce vascularized organ models without using sacrificial materials during fabrication. The high resolution of the method allows the one-step generation of various sophisticated hollow geometries. This sacrificial-free DIW (SF-DIW) approach is used to fabricate hepatic metastasis models of various cancer types and different formats for investigating the cytostatic activity of anti-cancer drugs. To this end, the models are incorporated into a newly developed perfusion system with integrated micropumps and an agar casting step that improves the physiological features of the bioprinted tissues. It is shown that the hepatic environment of the tumor models is capable of activating a prodrug, which inhibits breast cancer growth. This versatile SF-DIW approach is able to fabricate complicated perfusable constructs or microfluidic chips in a straightforward and cost-efficient manner. It can also be easily adapted to other cell types for generating vascularized organ tissues or cancer models that may support the development of new therapeutics.
BT - Advanced Functional Materials
DA - 2024
DO - 10.1002/adfm.202314171
IS - 30
LA - en
N2 - 3D bioprinting enables the fabrication of human organ models that can be used for various fields of biomedical research, including oncology and infection biology. An important challenge, however, remains the generation of vascularized, perfusable 3D models that closely simulate natural physiology. Here, a novel direct ink writing (DIW) approach is described that can produce vascularized organ models without using sacrificial materials during fabrication. The high resolution of the method allows the one-step generation of various sophisticated hollow geometries. This sacrificial-free DIW (SF-DIW) approach is used to fabricate hepatic metastasis models of various cancer types and different formats for investigating the cytostatic activity of anti-cancer drugs. To this end, the models are incorporated into a newly developed perfusion system with integrated micropumps and an agar casting step that improves the physiological features of the bioprinted tissues. It is shown that the hepatic environment of the tumor models is capable of activating a prodrug, which inhibits breast cancer growth. This versatile SF-DIW approach is able to fabricate complicated perfusable constructs or microfluidic chips in a straightforward and cost-efficient manner. It can also be easily adapted to other cell types for generating vascularized organ tissues or cancer models that may support the development of new therapeutics.
PY - 2024
EP - 2314171
T2 - Advanced Functional Materials
TI - Bioprinting of Perfusable Vascularized Organ Models for Drug Development via Sacrificial-Free Direct Ink Writing
UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.202314171
VL - 34
Y2 - 2025-11-20
SN - 1616-3028
ER -