TY - JOUR
KW - Biological models
KW - Drug screening
AU - Daisong Wang
AU - Remi Villenave
AU - Nadine Stokar-Regenscheit
AU - Hans Clevers
AB - Organoids are 3D structures derived from stem cells that recapitulate key architectural and functional aspects of the corresponding tissue. Compared with conventional 2D cell lines, human organoids provide experimental models that more closely reflect human physiology. Their ability to capture the complexity and heterogeneity of human tissues enables the study of disease mechanisms, drug efficacy and toxicity. When generated from patient material, organoids also allow the assessment of individual drug responses. In this Review, we explore the utility of organoids in drug discovery. We outline current methodologies for generating and maintaining organoids, examine their applications in disease modelling, drug screening and safety evaluation, and consider regulatory aspects and the challenges for their broader adoption in drug discovery.
BT - Nature Reviews Drug Discovery
DA - 2025-11-12
DO - 10.1038/s41573-025-01317-y
LA - en
N2 - Organoids are 3D structures derived from stem cells that recapitulate key architectural and functional aspects of the corresponding tissue. Compared with conventional 2D cell lines, human organoids provide experimental models that more closely reflect human physiology. Their ability to capture the complexity and heterogeneity of human tissues enables the study of disease mechanisms, drug efficacy and toxicity. When generated from patient material, organoids also allow the assessment of individual drug responses. In this Review, we explore the utility of organoids in drug discovery. We outline current methodologies for generating and maintaining organoids, examine their applications in disease modelling, drug screening and safety evaluation, and consider regulatory aspects and the challenges for their broader adoption in drug discovery.
PY - 2025
SP - 1
EP - 23
ST - Human organoids as 3D in vitro platforms for drug discovery
T2 - Nature Reviews Drug Discovery
TI - Human organoids as 3D in vitro platforms for drug discovery: opportunities and challenges
UR - https://www.nature.com/articles/s41573-025-01317-y
Y2 - 2025-11-13
SN - 1474-1784
ER -