TY - JOUR
KW - Biological techniques
KW - Computational biology and bioinformatics
KW - Neuroscience
KW - Stem cells
AU - Alexander G. Bury
AU - Alicja Olejnik
AU - Chiara Tocco
AU - Nathalie Saurat
AU - Elezabeth Stephen
AU - Dirk Hockemeyer
AU - Jens C. Schwamborn
AU - Lorenz Studer
AU - Pier Giorgio Mastroberardino
AU - Silvia Bolognin
AU - Tilo Kunath
AU - Viktor I. Korolchuk
AU - Janelle Drouin-Ouellet
AU - Heather Mortiboys
AB - Ageing is the primary risk factor for Parkinson’s disease, yet the intricate interplay between these processes remains ambiguous. This position paper, a collaborative output from the PD-AGE consortium, addresses the urgent need for standardising methods in in vitro modelling. A panel of international experts recommends human induced pluripotent stem cell (iPSC)-derived models, with chemically induced ageing methods, such as the SLO cocktail, as a robust system. Furthermore, the consortium highlights the value of direct and semi-direct reprogramming for retaining donor-specific ageing phenotypes. The paper also outlines a prioritised panel of measurable parameters, categorised into senescence, inflammaging, omics profiling, and mitochondrial dysfunction, providing a consistent framework to enhance research reproducibility, investigating the nexus of ageing and Parkinson’s. In addition, we provide links to SOPs (https://doi.org/10.5281/zenodo.15056603) [1] to measure the key measurable ageing parameters outlined in this review to facilitate consistency and reproducibility within the field.
BT - npj Parkinson's Disease
DA - 2025-10-09
DO - 10.1038/s41531-025-01137-2
IS - 1
LA - en
N2 - Ageing is the primary risk factor for Parkinson’s disease, yet the intricate interplay between these processes remains ambiguous. This position paper, a collaborative output from the PD-AGE consortium, addresses the urgent need for standardising methods in in vitro modelling. A panel of international experts recommends human induced pluripotent stem cell (iPSC)-derived models, with chemically induced ageing methods, such as the SLO cocktail, as a robust system. Furthermore, the consortium highlights the value of direct and semi-direct reprogramming for retaining donor-specific ageing phenotypes. The paper also outlines a prioritised panel of measurable parameters, categorised into senescence, inflammaging, omics profiling, and mitochondrial dysfunction, providing a consistent framework to enhance research reproducibility, investigating the nexus of ageing and Parkinson’s. In addition, we provide links to SOPs (https://doi.org/10.5281/zenodo.15056603) [1] to measure the key measurable ageing parameters outlined in this review to facilitate consistency and reproducibility within the field.
PY - 2025
EP - 289
ST - Investigating the ageing-Parkinson’s disease nexus
T2 - npj Parkinson's Disease
TI - Investigating the ageing-Parkinson’s disease nexus: standardisation of in vitro models and techniques by the PD-AGE network
UR - https://www.nature.com/articles/s41531-025-01137-2
VL - 11
Y2 - 2025-10-10
SN - 2373-8057
ER -