TY - JOUR
AU - Oscar J. Abilez
AU - Huaxiao Yang
AU - Yuan Guan
AU - Mengcheng Shen
AU - Zehra Yildirim
AU - Yan Zhuge
AU - Ravichandra Venkateshappa
AU - Shane R. Zhao
AU - Angello H. Gomez
AU - Marcel El-Mokahal
AU - Logan Dunkenberger
AU - Yoshikazu Ono
AU - Masafumi Shibata
AU - Peter N. Nwokoye
AU - Lei Tian
AU - Kitchener D. Wilson
AU - Evan H. Lyall
AU - Fangjun Jia
AU - Hung Ta Wo
AU - Gao Zhou
AU - Bryan Aldana
AU - Ioannis Karakikes
AU - Detlef Obal
AU - Gary Peltz
AU - Christopher K. Zarins
AU - Joseph C. Wu
AB - Although model organisms have provided insight into the earliest stages of cardiac and hepatic vascularization, we know very little about this process in humans because of ethical restrictions and the technical difficulty of obtaining embryos during very early development. In this study, we demonstrate that micropatterned human pluripotent stem cell–derived gastruloids enable in vitro modeling of the earliest stages of vascularization. We identify a combination of vascular-inducing factors that give rise to cardiac vascularized organoids with a spatially organized and branched vascular network. To show the broader utility of our vascularization strategy, we use the same vascular-inducing factors to produce hepatic vascularized organoids. Our results suggest that a conserved developmental program generates the vasculature within different types of organs.
BT - Science
DA - 2025-06-05
DO - 10.1126/science.adu9375
IS - 6751
N2 - Although model organisms have provided insight into the earliest stages of cardiac and hepatic vascularization, we know very little about this process in humans because of ethical restrictions and the technical difficulty of obtaining embryos during very early development. In this study, we demonstrate that micropatterned human pluripotent stem cell–derived gastruloids enable in vitro modeling of the earliest stages of vascularization. We identify a combination of vascular-inducing factors that give rise to cardiac vascularized organoids with a spatially organized and branched vascular network. To show the broader utility of our vascularization strategy, we use the same vascular-inducing factors to produce hepatic vascularized organoids. Our results suggest that a conserved developmental program generates the vasculature within different types of organs.
PY - 2025
EP - eadu9375
T2 - Science
TI - Gastruloids enable modeling of the earliest stages of human cardiac and hepatic vascularization
UR - https://www.science.org/doi/10.1126/science.adu9375
VL - 388
Y2 - 2025-10-02
ER -