TY - JOUR
KW - Adipocytes
KW - Fat-on-a-chip
KW - Glucose
KW - Glucose uptake
KW - Human-derived hydrogel
KW - Humans
KW - Lab-On-A-Chip Devices
KW - Lipolysis
KW - Obesity
KW - Three-dimensional cell culture
AU - Katie Hamel
AU - Emma Rogers
AU - Jeffrey M. Gimble
AU - Trivia Frazier
AU - Cecilia Sanchez
AB - Advancements in three-dimensional in vitro cultures pose a need for modification of established two-dimensional culture functional assay methods. Application of three-dimensional in vitro models in drug screening and target validation, specifically in the development of compounds targeting adipose metabolic activity, requires optimization of current glucose uptake and lipolysis assay protocols to effectively measure adipocyte function in a three-dimensional platform. This chapter describes the establishment of three-dimensional cultures using Obatala Sciences' human-derived hydrogel, maintenance and treatment of the cultures, and evaluation of compound response via lipolysis and glucose uptake assays.
BT - Methods in Molecular Biology (Clifton, N.J.)
DA - 2024
DO - 10.1007/978-1-0716-3762-3_23
LA - eng
N2 - Advancements in three-dimensional in vitro cultures pose a need for modification of established two-dimensional culture functional assay methods. Application of three-dimensional in vitro models in drug screening and target validation, specifically in the development of compounds targeting adipose metabolic activity, requires optimization of current glucose uptake and lipolysis assay protocols to effectively measure adipocyte function in a three-dimensional platform. This chapter describes the establishment of three-dimensional cultures using Obatala Sciences' human-derived hydrogel, maintenance and treatment of the cultures, and evaluation of compound response via lipolysis and glucose uptake assays.
PY - 2024
SP - 323
EP - 333
ST - Functional Assays in 3D ObaCell® Fat-on-a-Chip Cultures
T2 - Methods in Molecular Biology (Clifton, N.J.)
TI - Functional Assays in 3D ObaCell® Fat-on-a-Chip Cultures: Lipolysis and Glucose Uptake Assays
VL - 2783
SN - 1940-6029
ER -