TY - JOUR
AU - Marc Vila Cuenca
AU - Merve Bulut
AU - Christine L. Mummery
AU - Valeria V. Orlova
AB - Generation of functional vasculature within organoids is considered important for their development and maturation. However, direct differentiation of endothelial cells (ECs) in organoids remains challenging so that creating fully perfusable vasculature often still requires transplantation into host animals. This review discusses recent strategies for generating pre-vascularized human pluripotent stem cell (hPSC)-derived organoids, that include co-differentiation of ECs using growth factors or (an inducible transcription factor) ETV2, controlled assembly of tissue organoids with hPSC-derived ECs or Blood Vessel Organoids (BVOs), and 3D bioprinting. Additionally, the potential and key challenges of organ-on-chip technology for creating perfusable and functional vascular networks in organoids are explored, highlighting their implications for advancing research and improving experimental models of human tissue and disease.
BT - Current Opinion in Biomedical Engineering
DA - 2025-06-01
DO - 10.1016/j.cobme.2025.100586
N2 - Generation of functional vasculature within organoids is considered important for their development and maturation. However, direct differentiation of endothelial cells (ECs) in organoids remains challenging so that creating fully perfusable vasculature often still requires transplantation into host animals. This review discusses recent strategies for generating pre-vascularized human pluripotent stem cell (hPSC)-derived organoids, that include co-differentiation of ECs using growth factors or (an inducible transcription factor) ETV2, controlled assembly of tissue organoids with hPSC-derived ECs or Blood Vessel Organoids (BVOs), and 3D bioprinting. Additionally, the potential and key challenges of organ-on-chip technology for creating perfusable and functional vascular networks in organoids are explored, highlighting their implications for advancing research and improving experimental models of human tissue and disease.
PY - 2025
EP - 100586
ST - Vascularization of organoid microenvironments
T2 - Current Opinion in Biomedical Engineering
TI - Vascularization of organoid microenvironments: Perfusable networks for organoid growth and maturation
UR - https://www.sciencedirect.com/science/article/pii/S246845112500011X
VL - 34
Y2 - 2025-04-30
SN - 2468-4511
ER -