TY - JOUR
KW - Animal free medium
KW - cell culture
KW - Defined medium
KW - Ethically acceptable
KW - Human proteins
KW - Reproducible composition
AU - Tilo Weber
AU - Jeffrey Bajramovic
AU - Stina Oredsson
AB - Since 1958, cell culture media supplemented with fetal bovine serum is used, despite the well-known concerns about animal welfare, reproducibility, reliability, relevance, and safety. To obliterate these concerns and increase scientific accuracy, we recently published an open access, publicly available paper on a defined medium composition to make it possible for any lab to prepare this medium. The medium supports routine culturing and cell banking as well as investigations of growth curves, dose response testing of compounds of cells in 2D and 3D, and cell migration; all important aspects for research and toxicology. Here we give a detailed description of how to mix the defined universal cell culture medium in 14 simple steps to support any entity that wishes to make it. We also list different normal and cancer cell lines that have been cultured in the defined medium.•Open source composition of animal product free universal cell culture medium•Protocols for mixing solutions of small xeno free molecules for supplementation•Protocols for mixing solutions of human proteins for supplementation
BT - MethodsX
DA - 2024-06-01
DO - 10.1016/j.mex.2024.102592
N2 - Since 1958, cell culture media supplemented with fetal bovine serum is used, despite the well-known concerns about animal welfare, reproducibility, reliability, relevance, and safety. To obliterate these concerns and increase scientific accuracy, we recently published an open access, publicly available paper on a defined medium composition to make it possible for any lab to prepare this medium. The medium supports routine culturing and cell banking as well as investigations of growth curves, dose response testing of compounds of cells in 2D and 3D, and cell migration; all important aspects for research and toxicology. Here we give a detailed description of how to mix the defined universal cell culture medium in 14 simple steps to support any entity that wishes to make it. We also list different normal and cancer cell lines that have been cultured in the defined medium.•Open source composition of animal product free universal cell culture medium•Protocols for mixing solutions of small xeno free molecules for supplementation•Protocols for mixing solutions of human proteins for supplementation
PY - 2024
EP - 102592
T2 - MethodsX
TI - Preparation of a universally usable, animal product free, defined medium for 2D and 3D culturing of normal and cancer cells
UR - https://www.sciencedirect.com/science/article/pii/S2215016124000463
VL - 12
Y2 - 2025-04-30
SN - 2215-0161
ER -