TY - JOUR
KW - Cancer
KW - cell culture
KW - Cell-based assays
AU - Stina Oredsson
AU - Atena Malakpour-Permlid
AU - Johan Zhu
AU - Tilo Weber
AB - The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures. Here, we present a protocol for the use of OUR medium for routine culturing, cell banking, and medium modification for suspension culture. We describe steps for thawing, sub-culturing, and freezing cells. We then detail procedures for using these techniques for VERO, A549, THP-1, and Jurkat cells.
BT - STAR Protocols
DA - 2025-06-20
DO - 10.1016/j.xpro.2025.103781
IS - 2
N2 - The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures. Here, we present a protocol for the use of OUR medium for routine culturing, cell banking, and medium modification for suspension culture. We describe steps for thawing, sub-culturing, and freezing cells. We then detail procedures for using these techniques for VERO, A549, THP-1, and Jurkat cells.
PY - 2025
EP - 103781
T2 - STAR Protocols
TI - Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures
UR - https://www.sciencedirect.com/science/article/pii/S266616672500187X
VL - 6
Y2 - 2025-04-30
SN - 2666-1667
ER -