TY - JOUR
KW - Biotechnology and bioengineering
KW - Chemistry
KW - Material sciences
KW - Tissue engineering
AU - Tamilselvan Mohan
AU - Matej Bračič
AU - Doris Bračič
AU - Florian Lackner
AU - Chandran Nagaraj
AU - Andreja Dobaj Štiglic
AU - Rupert Kargl
AU - Karin Stana Kleinschek
AB - Three-dimensional (3D) and porous scaffolds made from nanocellulosic materials hold significant potential in tissue engineering (TE). Here, we present a protocol for fabricating self-standing (nano)cellulose-based 3D scaffolds designed for in vitro testing of cells from skin and cartilage tissues. We describe steps for preparation of nanocellulose ink, scaffold formation using 3D printing, and freeze-drying. We then detail post-processing procedures to enhance mechanical properties, stability, and biocompatibility. This protocol offers researchers a framework for developing versatile and sustainable biomaterials for regenerative medicine. For complete details on the use and execution of this protocol, please refer to Mohan et al.1 and Štiglic et al.2
BT - STAR Protocols
DA - 2025-03-21
DO - 10.1016/j.xpro.2024.103583
IS - 1
N2 - Three-dimensional (3D) and porous scaffolds made from nanocellulosic materials hold significant potential in tissue engineering (TE). Here, we present a protocol for fabricating self-standing (nano)cellulose-based 3D scaffolds designed for in vitro testing of cells from skin and cartilage tissues. We describe steps for preparation of nanocellulose ink, scaffold formation using 3D printing, and freeze-drying. We then detail post-processing procedures to enhance mechanical properties, stability, and biocompatibility. This protocol offers researchers a framework for developing versatile and sustainable biomaterials for regenerative medicine. For complete details on the use and execution of this protocol, please refer to Mohan et al.1 and Štiglic et al.2
PY - 2025
EP - 103583
T2 - STAR Protocols
TI - Protocol for the fabrication of self-standing (nano)cellulose-based 3D scaffolds for tissue engineering
UR - https://www.sciencedirect.com/science/article/pii/S2666166724007482
VL - 6
Y2 - 2025-04-03
SN - 2666-1667
ER -