02596nas a2200565   4500000000100000000000100001008004100002260001500043653002400058653002300082100001800105700002100123700002200144700002300166700001500189700002300204700001500227700002000242700001700262700001800279700002700297700001400324700001400338700002000352700002300372700002300395700002300418700002100441700002300462700001600485700002800501700002100529700002700550700001600577700002200593700002100615700001700636700001800653700002100671700001600692700002300708700002000731700002100751245009600772856005500868300000900923490000700932520107700939022001402016       2026                            d  c2026-09-2410aAlzheimer's disease10aDiagnostic markers1 aAram Aslanyan1 aSoumya Mukherjee1 aEleanor M. Moncur1 aAlicja Szadziewska1 aYingxin He1 aPrzemysław R. Kac1 aReid Coyle1 aElena Camporesi1 aChihiro Sato1 aNupur Ghoshal1 aNicolas R. Barthélemy1 aJack Wood1 aJunyue Ge1 aMaciej Dulewicz1 aSrinivas Koutarapu1 aKatherine Schwetye1 aKaleigh F. Roberts1 aEimear C. Murphy1 aFrancesco Carletti1 aKaj Blennow1 aFernando Gonzalez Ortiz1 aClaire A. Leckey1 aTatiana A. Giovannucci1 aKanza Tariq1 aHenrik Zetterberg1 aDonald L. Elbert1 aLewis Thorne1 aAhmed K. Toma1 aLaurence Watkins1 aNick C. Fox1 aRandall J. Bateman1 aJörg Hanrieder1 aRoss W. Paterson00aSpatiotemporal characterization of Alzheimer disease pathology in living human brain tissue  uhttps://www.nature.com/articles/s41467-026-76494-4  a98780 v173 aAlzheimer disease begins in the brain many years before symptoms, but early changes are usually studied after death or indirectly through fluid and imaging biomarkers. Here we show that small brain biopsies collected during ventriculoperitoneal shunt surgery can be used to study Alzheimer disease pathology and protein turnover during life. We analysed biopsies from 18 individuals with suspected normal pressure hydrocephalus, alongside matched ventricular and lumbar cerebrospinal fluid and post-mortem control brain tissue. Amyloid plaques were present in 9 of 18 biopsies, while mature tau tangles were rare. Matrix-assisted laser desorption/ionization mass spectrometry imaging mapped amyloid plaque chemistry within tissue. Phosphorylated tau 217 was enriched around plaques, supporting a local relationship between amyloid and tau pathology. Stable isotope labelling, which tracks newly made proteins, detected rapid tau labelling within approximately 3 hours and estimated brain tau half-life at about 34 days. Amyloid plaques showed little detectable turnover.  a2041-1723