02026nas a2200349   4500000000100000000000100001008004100002260001500043653001700058653002100075653002600096653001500122653001900137100002100156700001900177700001700196700001600213700002000229700001900249700001400268700002200282700002000304700001400324700002400338700001900362700002000381245007700401856005500478300000900533520112000542022001401662       2026                            d  c2026-07-2210aCell Lineage10aCellular imaging10aintestinal stem cells10aPhysiology10aRNA sequencing1 aKelli F. Johnson1 aXiangning Dong1 aYu-Hwai Tsai1 aAngeline Wu1 aSydney G. Clark1 aAbigail Vallie1 aSha Huang1 aCharlie J. Childs1 aRachel K. Zwick1 aIan Glass1 aKatherine D. Walton1 aOphir D. Klein1 aJason R. Spence00aMapping mesenchymal diversity in the human small intestine and organoids  uhttps://www.nature.com/articles/s41556-026-02027-2  a1-133 aThe organization of diverse mesenchymal populations during human small intestinal development is critical for tissue architecture and function yet remains poorly defined. Here, to construct a comprehensive, tissue-scale map of the developing human small intestine at single cell resolution, we leveraged single-cell RNA-sequencing data to build a Xenium spatial transcriptomics gene panel covering the cell diversity of the human small intestine. We defined five subpopulations occupying discrete anatomical locations within the lamina propria and submucosa—the subepithelial cells, lamina propria fibroblasts, submucosal fibroblasts, smooth muscle cells and CXCL13+ fibroblasts. Our data establish molecular markers to distinguish these populations in both sequencing and imaging data. We leverage this high-resolution atlas to interrogate cell–cell signalling, benchmark pluripotent stem cell-derived human intestinal organoids and to demonstrate how this resource can incorporate relative spatial organization into tissue analysis, with broad implications for modelling development, regeneration and disease.  a1476-4679