03234nas a2200745   4500000000100000000000100001008004100002260001500043653001700058653002100075653002100096653003000117100002100147700002400168700002000192700002500212700001700237700001800254700001700272700001900289700001900308700002000327700002600347700001900373700002600392700002200418700002000440700002000460700001900480700002300499700002400522700002400546700001800570700002600588700002000614700002000634700002000654700002700674700002200701700002100723700002000744700001500764700001800779700001900797700002400816700002300840700001800863700002300881700002000904700002200924700001900946700001600965700002100981700002001002700002701022700001201049700002101061700002801082700002401110245011201134856005501246300000901301520116401310022001402474       2026                            d  c2026-06-1510aCell biology10aCellular imaging10aImage processing10aStem-cell differentiation1 aCaroline Hookway1 aAntoine Borensztejn1 aLeigh K. Harris1 aTiffany Barszczewski1 aSara Carlson1 aGokhan Dalgin1 aSuraj Mishra1 aEllen M. Adams1 aJulie C. Dixon1 aRenske J. Dupar1 aJacqueline H. Edmonds1 aErik A. Ehlers1 aAlexandra J. Ferrante1 aMargaret A. Fuqua1 aClare R. Gamlin1 aPhilip Garrison1 aJanani Gopalan1 aBenjamin W. Gregor1 aMaxwell J. Hedayati1 aVictoria L. Hurless1 aKyle N. Klein1 aChantelle L. Leveille1 aSean L. Meharry1 aRicardo Mercado1 aHaley S. Morris1 aGouthamrajan Nadarajan1 aNivedita Nivedita1 aSandra A. Oluoch1 aSerge E. Parent1 aAmber Phan1 aBrock Roberts1 aAshwin Samudre1 aEmmanuel E. Sanchez1 aM. Filip Sluzewski1 aLev S. Snyder1 aDerek J. Thirstrup1 aHannah F. Thorp1 aJohn Paul Thottam1 aJulia R. Torvi1 aGaea Turman1 aMatheus P. Viana1 aLyndsay Wilhelm1 aChamari S. Wijesooriya1 aJie Yao1 aJulie A. Theriot1 aRuwanthi N. Gunawardane1 aSusanne M. Rafelski00aA human induced pluripotent stem cell model for the holistic study of epithelial-to-mesenchymal transitions  uhttps://www.nature.com/articles/s41592-026-03096-9  a1-133 aThe epithelial-to-mesenchymal transition (EMT) is a widely studied cell state change, yet differences in model design and measurement approaches limit comparison across studies. Addressing this challenge requires experimental model systems and analysis frameworks that support standardization across contexts. Here, we show that human induced pluripotent stem (hiPS) cells in defined cell culture geometries, two-dimensional colonies and three-dimensional lumenoids, enable multimodal measurements of EMT dynamics within a single experimental platform. Using fixed-cell and live-cell image-based assays, we quantify changes in cell migration, EMT-related molecular markers, cell–cell junction organization and interactions with the basement membrane, a specialized form of the extracellular matrix, during EMT induced in hiPS cells. We identify cell culture geometry-dependent differences in the timing of migration onset and show that basement membrane integrity can be quantitatively linked to these differences. Together, these results establish an imaging-based framework for analysis of cell state transitions and provide accessible datasets and tools.  a1548-7105