01943nas a2200265   4500000000100000008004100001260001500042100002400057700002200081700002300103700001900126700002600145700001700171700002200188700002100210700002000231700002000251700001800271700002200289245013800311856005500449300001300504490000700517520115300524       2026                            d  c2026-04-031 aKatherine M. Nelson1 aDaniel J. Minahan1 aVonetta L. Edwards1 aIan J. Glomski1 aDavid J. Delgado Diaz1 aKeena Thomas1 aForrest C. Walker1 aPatrik M. Bavoil1 aIsabelle Derré1 aAlison K. Criss1 aJacques Ravel1 aJason P. Gleghorn00aA microphysiologic human cervical model recapitulates microbial, immune, and pathogenic properties of sexually transmitted infections  uhttps://www.science.org/doi/10.1126/sciadv.aeb4864  aeaeb48640 v123 aSexually transmitted infections (STIs) of the cervicovaginal mucosa are among the most common global infections. Current monolayer cell culture and animal models fail to reproduce the multilevel complexity required to investigate host-microbiota-pathogen relationships simultaneously and/or with sufficient physiological relevance. To address this limitation, we have developed a microphysiologic system (MPS) that models human cervical tissue and its microbiota and is susceptible to infection by two prominent genital pathogens, Chlamydia trachomatis and Neisseria gonorrhoeae. Notably, this MPS platform recapitulates essential dynamic, polymicrobial, immune, and pathogenic features of chlamydial and gonococcal infections as they occur in humans. The low-cost MPS device requires no specialized equipment or specific expertise and was experimentally validated across multiple nonengineering, remotely located laboratories, demonstrating its transferability and reproducibility. The platform provides a tool for research into genital infections in a system that closely mimics the cervical epithelium, an important advance over existing models.