02416nas a2200301   4500000000100000000000100001008004100002653001900043653003300062653004500095653003000140653002100170100001500191700001900206700002500225700001900250700001900269700002000288700002400308700001800332700001800350245009800368856006700466300001100533490000800544520154800552022001402100                                       d10aairway-on-chip10aair–liquid interface (ALI)10aengineered extracellular matrix hydrogel10amelt electrowriting (MEW)10atubular geometry1 aAli Doryab1 aJack F. Murphy1 aMichael Bartolf-Kopp1 aJenny C-C Hsin1 aAlice Chernaik1 aFrank McCaughan1 aYan Yan Shery Huang1 aTomasz Jungst1 aJürgen Groll00aHydrogel-Based Airway-on-Tube With Perfusable Endothelial Lumen and Outward Epithelialization  uhttps://onlinelibrary.wiley.com/doi/abs/10.1002/adma.202523588  ae235880 vn/a3 aChronic lung diseases are a leading cause of mortality worldwide, yet therapeutic options remain limited. A major barrier to pulmonary drug development is the lack of preclinical models that recapitulate lung complexity. While recent airway-on-chip models have advanced by integrating vascular and extracellular matrix (ECM) components, these are largely limited to planar configurations. Only a few tubular designs exist, yet they generally lack a perfusable vascular compartment that supports dynamic endothelial-epithelial interactions. To address this gap, we introduce an airway-on-tube that integrates an engineered ECM (EnECM) hydrogel, tuned to match lung tissue stiffness, with a tubular melt electrowritten (MEW) scaffold. The MEW reinforces the EnECM hydrogel for dynamic culture without affecting cell behavior. The tubular EnECM/MEW construct incorporates patient-derived primary human lung microvascular endothelial cells embedded within the EnECM hydrogel, forming a perfusable endothelial lumen, while primary human bronchial epithelial cells are cultured on the outer (abluminal) surface, establishing an outward-facing epithelium at the air-liquid interface (ALI). Pulsatile perfusion through the endothelial lumen delivers nutrients and mechanical cues (shear stress and cyclic stretch) while maintaining ALI culture. Together, this study establishes a versatile hydrogel-based platform for next-generation airway-on-chip models, opening new opportunities for preclinical lung research and precision therapeutic development.  a1521-4095