01378nas a2200277   4500000000100000000000100001008004100002260001500043653001700058653002200075653002200097653001400119653002400133653001600157653001500173100002100188700002200209700001900231700002600250700002800276245013000304300001100434490000600445520063500451022001401086       2025                            d  c2025-12-2410aCell biology10aCell-based assays10aMolecular biology10aorganoids10aSignal Transduction10aSingle cell10aStem cells1 aKari-Pekka Skarp1 aBahar Yetkin-Arik1 aSuze A. Jansen1 aCaroline A. Lindemans1 aMagdalena J. Lorenowicz00aProtocol for modeling the repair of intestinal damage by co-culturing mesenchymal stromal/stem cells and intestinal organoids  a1042920 v73 aMesenchymal stem/stromal cells (MSCs) are known for their regenerative properties. This protocol describes a co-culture system for investigating molecular interactions between MSCs and intestinal epithelial organoids following injury. We outline steps for assessing the immediate effects of MSCs on organoid growth and survival, as well as a model for evaluating longer term responses. The workflow is adaptable and can be readily modified to examine MSC interactions with additional cell types or in different injury contexts. For complete information on the use and execution of this protocol, please refer to Yetkin-Arik et al.  a2666-1667