01778nas a2200301   4500000000100000000000100001008004100002260001500043653001500058653002500073100001600098700002100114700002000135700002100155700001800176700001800194700001800212700002000230700001700250700001900267700002100286245008700307856005500394300000600449490000600455520100100461022001401462       2025                            d  c2025-01-2910aPhysiology10aReproductive biology1 aOla Gutzeit1 aAakanksha Gulati1 aZohreh Izadifar1 aAnna Stejskalova1 aHassan Rhbiny1 aJustin Cotton1 aBogdan Budnik1 aSanjid Shahriar1 aGirija Goyal1 aAbidemi Junaid1 aDonald E. Ingber00aCervical mucus in linked human Cervix and Vagina Chips modulates vaginal dysbiosis  uhttps://www.nature.com/articles/s44294-025-00054-2  a50 v33 aThis study explores the protective role of cervicovaginal mucus in maintaining vaginal health, particularly in relation to bacterial vaginosis (BV), using organ chip technology. By integrating human Cervix and Vagina Chips, we demonstrated that cervical mucus significantly reduces inflammation and epithelial damage caused by a dysbiotic microbiome commonly associated with BV. Proteomic analysis of the Vagina Chip, following exposure to mucus from the Cervix Chip, revealed differentially abundant proteins, suggesting potential biomarkers and therapeutic targets for BV management. Our findings highlight the essential function of cervical mucus in preserving vaginal health and underscore the value of organ chip models for studying complex interactions within the female reproductive tract. This research provides new insights into the mechanisms underlying vaginal dysbiosis and opens avenues for developing targeted therapies and diagnostic tools to enhance women’s reproductive health.  a2948-1716