01981nas a2200313   4500000000100000008004100001260001500042653001600057653002000073653001800093653001000111100001400121700001400135700001000149700001200159700001600171700001600187700001600203700001200219700001600231700001800247700001600265245008500281856007200366300001100438490000800449520119600457022001401653       2022                            d  c2022-05-1510aCervix Chip10aHyaluronic acid10amicrofluidics10aSperm1 aSai-Xi Yu1 aYanan Liu1 aYi Wu1 aHao Luo1 aRufei Huang1 aYa-Jun Wang1 aXuemei Wang1 aHai Gao1 aHuijuan Shi1 aGuangyin Jing1 aYan-Jun Liu00aCervix chip mimicking cervical microenvironment for quantifying sperm locomotion  uhttps://www.sciencedirect.com/science/article/pii/S095656632200080X  a1140400 v2043 aAs the gate for sperm swimming into the female reproductive tract, cervix is full of cervical mucus, which plays an important role in sperm locomotion. The fact that sperm cannot pass through the cervical mucus-cervix microenvironment will cause the male infertility. However, how the sperm swim across the cervix microenvironment remains elusive. We used hyaluronic acid (HA), a substitute of cervical mucus to mimic cervix microenvironment and designed a cervix chip to study sperm selection and behavior. An accumulation of sperm in HA confirmed that HA served as a reservoir for sperm, similar to cervical mucus. We found that sperm escaping from HA exhibited higher motility than the sperm accessing into HA, suggesting that HA functions as a filter to select sperm with high activity. Our findings construct a practical platform to explore the sophisticated interaction of sperm with cervix microenvironment, with elaborate swimming indicators thus provide a promising cervix chip for sperm selection with kinematic features on-demand. What's more, the cervix chip allows the convenient use in clinical infertility diagnosis, owing to the advantage of simple, fast and high efficiency.  a0956-5663