02539nas a2200313   4500000000100000000000100001008004100002653003300043653002700076653002400103653003600127653002700163653002200190100002000212700001900232700002600251700002300277700001900300700002300319700002000342700001800362700001700380245011400397856006700511300001100578490000800589520161400597022001402211                                       d10a3D neural tissue engineering10abicontinuous scaffolds10abioactive materials10ahierarchical microarchitectures10ain vitro tissue models10aNeural Stem Cells1 aPrince D. Okoro1 aKevin Dalsania1 aShiril B. Iragavarapu1 aBenjamin Dela Cruz1 aAihik Banerjee1 aMerve Basaranbilek1 aMartin F. Haase1 aBahman Anvari1 aIman Noshadi00aBicontinuous Microarchitected Scaffolds Provide Topographic Cues That Govern Neuronal Behavior and Maturation  uhttps://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.202509452  ae094520 vn/a3 a3D tissue-engineered models hold great promise for recreating the intricate architecture and dynamic functions of neural tissues. However, replicating the nuanced structural cues of the brain in vitro remains challenging, as existing platforms often fail to capture the precise architectural motifs that regulate biological responses. Here, a bicontinuous interfacially jammed emulsion gel (bijel)-based fabrication strategy that combines solvent transfer-induced phase separation (STrIPS), microfluidics, and bioprinting to develop a Bijel-Integrated PORous Engineered System (BIPORES) for neural tissue engineering is introduced. This multifaceted approach yields scaffolds featuring interconnected micropores and textured surfaces interspersed with a hyperbolic curvature, seamlessly integrated within macroscale fibrous networks. By leveraging STrIPS of a ternary precursor mixture stabilized by amphiphilic nanoparticles, we synthesized poly(ethylene glycol) diacrylate (PEGDA) BIPORES support neural stem cell adhesion within 30 s without additional biological factors—a first for PEGDA scaffolds. Long-term cultures demonstrate extensive migration, robust proliferation, and differentiation into neuronal and astrocytic lineages, forming 3D networks with enhanced synaptic activity. Collagen encapsulation amplifies 3D cell growth, simulating native neuroanatomical compartmentalization. From a biomimicry standpoint, this multiscale fabrication strategy better approximates native neural tissue dynamics with significant implications for disease modeling, drug screening, and regenerative therapies.  a1616-3028