02053nas a2200313   4500000000100000000000100001008004100002260000900043653001600052653001400068653002500082653001500107653001700122653002100139653001300160653001900173653001400192100002000206700002100226700002100247700001600268700002100284245013300305856006300438300001100501490000700512520120600519022001401725       2025                            d  c202510aCervix Chip10afertility10ahuman in vitro model10amicrobiome10amicrofluidic10amucosal immunity10apathogen10apre-term birth10aPregnancy1 aZohreh Izadifar1 aAnna Stejskalova1 aAakanksha Gulati1 aOla Gutzeit1 aDonald E. Ingber00aHuman Cervix Chip: A Preclinical Model for Studying the Role of the Cervical Mucosa and Microbiome in Female Reproductive Health  uhttps://onlinelibrary.wiley.com/doi/abs/10.1002/bies.70014  ae700140 v473 aAdvancements in women's reproductive health have been hindered by insufficient knowledge and the underrepresentation of women in research, leading to symptom-focused care with poor outcomes. Modeling female reproductive biology and disease pathophysiology has been challenging due to the complexity and dynamic nature of the female organs. Here, we briefly review recent advancements made with a new in vitro microfluidic organ-on-a-chip model of the human cervix (Cervix Chip) that faithfully mimics key features of the cervix, including mucus production and physiological responses to hormonal, environmental, and microbial stimuli. We also discuss how this preclinical platform can provide a way to obtain unique insights into the role of mucosal immunity, genetic and risk factors, as well as microbiome and pathogen interactions in human cervix health and disease, while bridging knowledge gaps in fertility and pregnancy-related conditions. By enabling preclinical drug screening and accelerating translational research, the Cervix Chip holds the potential to improve the development of therapeutics, diagnostics, and ultimately, the sexual and reproductive health of millions of women globally.  a1521-1878