02153nas a2200361   4500000000100000000000100001008004100002260001500043653002600058653004500084653001700129653001500146100002200161700001900183700001700202700002000219700002200239700002000261700002300281700001800304700003300322700002000355700001600375700002400391700002700415700002200442245013000464856005500594300000800649490000700657520111300664022001401777       2025                            d  c2025-10-0910aBiological techniques10aComputational biology and bioinformatics10aNeuroscience10aStem cells1 aAlexander G. Bury1 aAlicja Olejnik1 aChiara Tocco1 aNathalie Saurat1 aElezabeth Stephen1 aDirk Hockemeyer1 aJens C. Schwamborn1 aLorenz Studer1 aPier Giorgio Mastroberardino1 aSilvia Bolognin1 aTilo Kunath1 aViktor I. Korolchuk1 aJanelle Drouin-Ouellet1 aHeather Mortiboys00aInvestigating the ageing-Parkinson’s disease nexus: standardisation of in vitro models and techniques by the PD-AGE network  uhttps://www.nature.com/articles/s41531-025-01137-2  a2890 v113 aAgeing is the primary risk factor for Parkinson’s disease, yet the intricate interplay between these processes remains ambiguous. This position paper, a collaborative output from the PD-AGE consortium, addresses the urgent need for standardising methods in in vitro modelling. A panel of international experts recommends human induced pluripotent stem cell (iPSC)-derived models, with chemically induced ageing methods, such as the SLO cocktail, as a robust system. Furthermore, the consortium highlights the value of direct and semi-direct reprogramming for retaining donor-specific ageing phenotypes. The paper also outlines a prioritised panel of measurable parameters, categorised into senescence, inflammaging, omics profiling, and mitochondrial dysfunction, providing a consistent framework to enhance research reproducibility, investigating the nexus of ageing and Parkinson’s. In addition, we provide links to SOPs (https://doi.org/10.5281/zenodo.15056603) [1] to measure the key measurable ageing parameters outlined in this review to facilitate consistency and reproducibility within the field.  a2373-8057