01049nas a2200217   4500000000100000000000100001008004100002260001200043653002200055653002100077653002000098100002400118700002200142700001400164245008800178856005500266300001200321490000600333520047800339022001400817       2025                            d  c2025-0510aCircadian rhythms10aDrug development10aDrug regulation1 aAlastair G. Stewart1 aAvanka Gunatilaka1 aBryan Gao00aShould culture media for microphysiological systems be nutritionally physiological?  uhttps://www.nature.com/articles/s44222-025-00290-w  a357-3580 v33 aThe incipient use of microphysiological systems (MPSs) in the drug regulatory pathway is based on equivalent or superior predictive value in the evaluation of drug efficacy, safety and toxicology, compared with other novel alternative methods (NAMs) or animal studies. Current MPS practice is not deploying available physiological culture media solutions. Will the clinical translational reliability of MPSs be potentially compromised by the use of hyper-nutritional media?  a2731-6092