01496nas a2200313   4500000000100000000000100001008004100002260000900043653001500052653001800067653001200085653001900097653002700116653001100143653002600154653001400180653001200194653003500206100001600241700001600257700002200273700001900295700002000314245009800334300001200432490000900444520071500453022001401168       2024                            d  c202410aAdipocytes10aFat-on-a-chip10aGlucose10aGlucose uptake10aHuman-derived hydrogel10aHumans10aLab-On-A-Chip Devices10aLipolysis10aObesity10aThree-dimensional cell culture1 aKatie Hamel1 aEmma Rogers1 aJeffrey M. Gimble1 aTrivia Frazier1 aCecilia Sanchez00aFunctional Assays in 3D ObaCell® Fat-on-a-Chip Cultures: Lipolysis and Glucose Uptake Assays  a323-3330 v27833 aAdvancements in three-dimensional in vitro cultures pose a need for modification of established two-dimensional culture functional assay methods. Application of three-dimensional in vitro models in drug screening and target validation, specifically in the development of compounds targeting adipose metabolic activity, requires optimization of current glucose uptake and lipolysis assay protocols to effectively measure adipocyte function in a three-dimensional platform. This chapter describes the establishment of three-dimensional cultures using Obatala Sciences' human-derived hydrogel, maintenance and treatment of the cultures, and evaluation of compound response via lipolysis and glucose uptake assays.  a1940-6029