01133nas a2200217   4500000000100000008004100001260001500042653001100057653001700068653002200085100001900107700002800126700001400154700001500168245014200183856007200325300001100397490000600408520048700414022001400901       2025                            d  c2025-06-2010aCancer10acell culture10aCell-based assays1 aStina Oredsson1 aAtena Malakpour-Permlid1 aJohan Zhu1 aTilo Weber00aProtocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures  uhttps://www.sciencedirect.com/science/article/pii/S266616672500187X  a1037810 v63 aThe Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures. Here, we present a protocol for the use of OUR medium for routine culturing, cell banking, and medium modification for suspension culture. We describe steps for thawing, sub-culturing, and freezing cells. We then detail procedures for using these techniques for VERO, A549, THP-1, and Jurkat cells.  a2666-1667