01476nas a2200169   4500000000100000008004100001260001500042100001800057700001800075700001600093700001600109245012500125856005200250300001400302490000700316520098300323       2024                            d  c2024-12-091 aDae-Seop Shin1 aMyung Jin Son1 aMyungae Bae1 aHyunwoo Kim00aLocal Stiffness Measurement of Hepatic Steatosis Model Liver Organoid by Fluorescence Imaging-Assisted Probe Indentation  uhttps://doi.org/10.1021/acsbiomaterials.4c01242  a7386-73930 v103 aMechanical stiffness of liver organoid is a key indicator for the progress of hepatic steatosis. Probe indentation is a noninvasive methodology to measure Young’s modulus (YM); however, the inhomogeneous nature of the liver organoid induces measurement uncertainty requiring a large number of indentations covering a wide scanning area. Here, we demonstrate that lipid-stained fluorescence imaging-assisted probe indentation significantly reduces the number of measurements by specifying the highly lipid-induced area. Lipid-stained hepatic steatosis model liver organoid shows broad fluorescence distributions that are spatially correlated with a decreased YM on a lipid-filled region with bright fluorescence compared with that measured on a blank region with dark fluorescence. The organoid viability remained robust even after exposure to an ambient condition up to 6 h, showing that probe indentations can be noninvasive methods for liver organoid stiffness measurements.