@article{bibcite_9086,
  keywords = {Cellular neuroscience, Neuronal development},
  author = {Irene Faravelli and Noelia Ant{\'o}n-Bola{\~n}os and Anqi Wei and Tyler Faits and Abhishek Sampath Kumar and Sophia Andreadis and Rahel Kastli and Marta Montero Crespo and Mara Steiger and Daniel Leible and Elizabeth Zhang and Bobae An and Yaron Meirovitch and Sayara Silwal and Sung Min Yang and Alexander Kovacsovics and Xian Adiconis and Helene Kretzmer and Joshua Z. Levin and Edward S. Boyden and Jeff Lichtman and Aviv Regev and Alexander Meissner and Paola Arlotta},
  title = {Human brain organoids record the passage of time over multiple years},
  abstract = {The human brain develops and matures over an exceptionally prolonged period of time that spans nearly two decades of life. Processes that govern species-specific aspects of human postnatal brain development are difficult to study in animal models1. While human brain organoids offer a promising in vitro model, they have thus far been shown to largely mimic early stages of brain development. Here we develop human brain organoids for 5 years in culture, optimizing growth conditions to extend excitatory neuron viability beyond previous limits. Using maturation-associated modules derived from endogenous human brain, we show that brain organoids transcriptionally age with cell type specificity over years in culture. Whole-genome methylation profiling reveals that the predicted epigenomic age of organoids correlates precisely with time spent in vitro, and parallels epigenomic ageing in vivo. Notably, we show that in chimeric organoids generated by mixing neural progenitors of different ages, old progenitors rapidly produce late neuronal fates, skipping the production of earlier neuronal progeny, therefore showing that progenitors that age in organoids retain a memory of the time spent in vitro. The data indicate that human brain organoids can continue to mature and record the passage of time over many years in culture.},
  year = {2026},
  journal = {Nature},
  pages = {1-11},
  month = {2026-08-19},
  issn = {1476-4687},
  url = {https://www.nature.com/articles/s41586-026-10877-x},
  doi = {10.1038/s41586-026-10877-x},
  language = {en},
}