@article{bibcite_8701,
  keywords = {Ebola virus, Viral pathogenesis, Virus{\textendash}host interactions},
  author = {Lina Widerspick and Santiago Vidal Freire and Johanna F. Steffen and Shruti Shirsathe and Petra Allartz and Molly A. Vickers and Christoph Henkel and Pedro Neira Pel{\'e}n and Julia Nave and Monika Rottstegge and Michelle Heung and Stephanie Wurr and Dennis Tappe and Lisa Oestereich and Jonas M{\"u}ller and Angelique Hoelzemer and Leonore Mensching and Thomas Hoenen and Nicole C. Kleinstreuer and Ian Crozier and John G. Bernbaum and Gabriella Worwa and Jens H. Kuhn and Gustavo Palacios and C{\'e}sar Mu{\~n}oz-Fontela},
  title = {Host{\textendash}virus determinants of Ebola virus persistence in a human cerebral organoid model},
  abstract = {Ebola virus (EBOV) causes Ebola virus disease (EVD), a multisystemic human disease associated with an extraordinarily high case-fatality rate. EVD survivors may experience recrudescent inflammation with viral persistence in immune-privileged tissues, including the central nervous system (CNS). Persistence, defined by ongoing replication of the EBOV genome beyond the acute disease phase, may lead to virion production. Productive persistence has been linked to re-initiation of EVD outbreaks. We developed a human cerebral organoid model to investigate the host and viral determinants of EBOV CNS persistence. In this model, EBOV persistence for 120 days was sustained by continuous infection of astrocytes and neurons and the recruitment and infection of microglia. This was accompanied by the emergence of EBOV defective viral genomes and genomic subvariants, cell-to-cell transmission, activation of cell-specific innate immunity, and late brain organoid inflammation, indicating that persistent infection of EBOV within immune-privileged niches drives local inflammation.},
  year = {2026},
  journal = {Nature Microbiology},
  pages = {1-16},
  month = {2026-06-12},
  issn = {2058-5276},
  url = {https://www.nature.com/articles/s41564-026-02388-2},
  doi = {10.1038/s41564-026-02388-2},
  language = {en},
}