@article{bibcite_8491,
  keywords = {Cell biology, Cellular imaging, Image processing, Stem-cell differentiation},
  author = {Caroline Hookway and Antoine Borensztejn and Leigh K. Harris and Tiffany Barszczewski and Sara Carlson and Gokhan Dalgin and Suraj Mishra and Ellen M. Adams and Julie C. Dixon and Renske J. Dupar and Jacqueline H. Edmonds and Erik A. Ehlers and Alexandra J. Ferrante and Margaret A. Fuqua and Clare R. Gamlin and Philip Garrison and Janani Gopalan and Benjamin W. Gregor and Maxwell J. Hedayati and Victoria L. Hurless and Kyle N. Klein and Chantelle L. Leveille and Sean L. Meharry and Ricardo Mercado and Haley S. Morris and Gouthamrajan Nadarajan and Nivedita Nivedita and Sandra A. Oluoch and Serge E. Parent and Amber Phan and Brock Roberts and Ashwin Samudre and Emmanuel E. Sanchez and M. Filip Sluzewski and Lev S. Snyder and Derek J. Thirstrup and Hannah F. Thorp and John Paul Thottam and Julia R. Torvi and Gaea Turman and Matheus P. Viana and Lyndsay Wilhelm and Chamari S. Wijesooriya and Jie Yao and Julie A. Theriot and Ruwanthi N. Gunawardane and Susanne M. Rafelski},
  title = {A human induced pluripotent stem cell model for the holistic study of epithelial-to-mesenchymal transitions},
  abstract = {The epithelial-to-mesenchymal transition (EMT) is a widely studied cell state change, yet differences in model design and measurement approaches limit comparison across studies. Addressing this challenge requires experimental model systems and analysis frameworks that support standardization across contexts. Here, we show that human induced pluripotent stem (hiPS) cells in defined cell culture geometries, two-dimensional colonies and three-dimensional lumenoids, enable multimodal measurements of EMT dynamics within a single experimental platform. Using fixed-cell and live-cell image-based assays, we quantify changes in cell migration, EMT-related molecular markers, cell{\textendash}cell junction organization and interactions with the basement membrane, a specialized form of the extracellular matrix, during EMT induced in hiPS cells. We identify cell culture geometry-dependent differences in the timing of migration onset and show that basement membrane integrity can be quantitatively linked to these differences. Together, these results establish an imaging-based framework for analysis of cell state transitions and provide accessible datasets and tools.},
  year = {2026},
  journal = {Nature Methods},
  pages = {1-13},
  month = {2026-06-15},
  issn = {1548-7105},
  url = {https://www.nature.com/articles/s41592-026-03096-9},
  doi = {10.1038/s41592-026-03096-9},
  language = {en},
}