@article{bibcite_7791,
  keywords = {WNT7A, endometrium, Epithelial Cells, luminal epithelium, Menstrual Cycle, menstruation, organoids, Regeneration, Transcriptomics, wound healing},
  author = {Konstantina Nikolakopoulou and Weand Yba{\~n}ez and Lh{\'e}anna Klaeyl{\'e} and Lisa Frugoli and Tereza Cindrova-Davies and Hans-Rudolf Hotz and Charlotte Soneson and Margherita Yayoi Turco},
  title = {An <i>in vitro</i> menstrual cycle using organoids captures epithelial cell transitions during menstruation and regeneration of the human endometrium},
  abstract = {Menstruation is an unusual process in which the human endometrium undergoes cyclical shedding with scarless regeneration. Despite its pivotal role in reproductive health, the cellular states and interactions orchestrating this process remain poorly defined, largely due to the lack of in vitro systems that capture the inaccessible perimenstrual window. We use human endometrial organoids to establish an in vitro menstrual cycle (IVMC) protocol that recapitulates cyclical epithelial dynamics. We validate the IVMC by benchmarking against in vivo samples spanning the menstrual window through histology, transcriptomic, and multiplex secreted-protein analysis. During menstruation, the in vivo luminal epithelium acquires a distinct transcriptomic signature, characterized by WNT7A expression. Loss of WNT7A compromises long-term organoid survival, highlighting its functional importance. The regeneration-associated luminal epithelium acts as a signaling hub during regeneration through interactions with the vasculature. This work opens new avenues to dissect the unique regenerative program of the endometrium in health and disease.},
  year = {2026},
  journal = {Cell Stem Cell},
  month = {2026-04-28},
  issn = {1934-5909},
  url = {https://www.sciencedirect.com/science/article/pii/S1934590926001451},
  doi = {10.1016/j.stem.2026.04.005},
}