@article{bibcite_7371,
  keywords = {airway-on-chip, air{\textendash}liquid interface (ALI), engineered extracellular matrix hydrogel, melt electrowriting (MEW), tubular geometry},
  author = {Ali Doryab and Jack F. Murphy and Michael Bartolf-Kopp and Jenny C-C Hsin and Alice Chernaik and Frank McCaughan and Yan Yan Shery Huang and Tomasz Jungst and J{\"u}rgen Groll},
  title = {Hydrogel-Based Airway-on-Tube With Perfusable Endothelial Lumen and Outward Epithelialization},
  abstract = {Chronic lung diseases are a leading cause of mortality worldwide, yet therapeutic options remain limited. A major barrier to pulmonary drug development is the lack of preclinical models that recapitulate lung complexity. While recent airway-on-chip models have advanced by integrating vascular and extracellular matrix (ECM) components, these are largely limited to planar configurations. Only a few tubular designs exist, yet they generally lack a perfusable vascular compartment that supports dynamic endothelial-epithelial interactions. To address this gap, we introduce an airway-on-tube that integrates an engineered ECM (EnECM) hydrogel, tuned to match lung tissue stiffness, with a tubular melt electrowritten (MEW) scaffold. The MEW reinforces the EnECM hydrogel for dynamic culture without affecting cell behavior. The tubular EnECM/MEW construct incorporates patient-derived primary human lung microvascular endothelial cells embedded within the EnECM hydrogel, forming a perfusable endothelial lumen, while primary human bronchial epithelial cells are cultured on the outer (abluminal) surface, establishing an outward-facing epithelium at the air-liquid interface (ALI). Pulsatile perfusion through the endothelial lumen delivers nutrients and mechanical cues (shear stress and cyclic stretch) while maintaining ALI culture. Together, this study establishes a versatile hydrogel-based platform for next-generation airway-on-chip models, opening new opportunities for preclinical lung research and precision therapeutic development.},
  journal = {Advanced Materials},
  volume = {n/a},
  pages = {e23588},
  issn = {1521-4095},
  url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.202523588},
  doi = {10.1002/adma.202523588},
  language = {en},
}