@article{bibcite_6776,
  keywords = {Adipocytes, White, Adipose Tissue, White, Adrenergic beta-Agonists, Animals, Biological Transport, Culture Media, Dimethylpolysiloxanes, Fatty Acids, Humans, Isoproterenol, L-Lactate Dehydrogenase, Lab-On-A-Chip Devices, Microtechnology, Models, Biological, Obesity, Primary Cell Culture},
  author = {Julia Rogal and Carina Binder and Elena Kromidas and Julia Roosz and Christopher Probst and Stefan Schneider and Katja Schenke-Layland and Peter Loskill},
  title = {WAT-on-a-chip integrating human mature white adipocytes for mechanistic research and pharmaceutical applications},
  abstract = {Obesity and its numerous adverse health consequences have taken on global, pandemic proportions. White adipose tissue (WAT) - a key contributor in many metabolic diseases - contributes about one fourth of a healthy human{\textquoteright}s body mass. Despite its significance, many WAT-related pathophysiogical mechanisms in humans are still not understood, largely due to the reliance on non-human animal models. In recent years, Organ-on-a-chip (OoC) platforms have developed into promising alternatives for animal models; these systems integrate engineered human tissues into physiological microenvironment supplied by a vasculature-like microfluidic perfusion. Here, we report the development of a novel OoC that integrates functional mature human white adipocytes. The WAT-on-a-chip is a multilayer device that features tissue chambers tailored specifically for the maintenance of 3D tissues based on human primary adipocytes, with supporting nourishment provided through perfused media channels. The platform{\textquoteright}s capability to maintain long-term viability and functionality of white adipocytes was confirmed by real-time monitoring of fatty acid uptake, by quantification of metabolite release into the effluent media as well as by an intact responsiveness to a therapeutic compound. The novel system provides a promising tool for wide-ranging applications in mechanistic research of WAT-related biology, in studying of pathophysiological mechanisms in obesity and diabetes, and in R\&D of pharmaceutical industry.},
  year = {2020},
  journal = {Scientific Reports},
  volume = {10},
  pages = {6666},
  month = {2020-04-20},
  issn = {2045-2322},
  doi = {10.1038/s41598-020-63710-4},
  language = {eng},
}