@article{6431,
  keywords = {Biomedical Engineering, Biophysical models},
  author = {Adrià Casamitjana and Matteo Mancini and Eleanor Robinson and Loïc Peter and Roberto Annunziata and Juri Althonayan and Shauna Crampsie and Emily Blackburn and Benjamin Billot and Alessia Atzeni and Oula Puonti and Yaël Balbastre and Peter Schmidt and James Hughes and Jean C. Augustinack and Brian L. Edlow and Lilla Zöllei and David L. Thomas and Dorit Kliemann and Martina Bocchetta and Catherine Strand and Janice L. Holton and Zane Jaunmuktane and Juan Eugenio Iglesias},
  title = {A probabilistic histological atlas of the human brain for MRI segmentation},
  abstract = {In human neuroimaging, brain atlases are essential for segmenting regions of interest (ROIs) and comparing subjects in a common coordinate frame. State-of-the-art atlases derived from histology1–3 provide exquisite three-dimensional cytoarchitectural maps but lack probabilistic labels throughout the whole brain: that is, the likelihood of each location belonging to a given ROI. Here we present NextBrain, a probabilistic histological atlas of the whole human brain. We developed artificial intelligence-enabled methods to align roughly 10,000 histological sections from five whole brain hemispheres into three-dimensional volumes and to produce delineations for 333 ROIs on these sections. We also created a companion Bayesian tool for automatic segmentation of these ROIs in magnetic resonance imaging (MRI) scans. We showcase two applications of the atlas: segmentation of ultra-high-resolution ex vivo MRI and volumetric analysis of Alzheimer’s disease using in vivo MRI. We publicly release raw and aligned data, an online visualization tool, the atlas, the segmentation tool, and ground truth delineations for a high-resolution ex vivo hemisphere used in validation. By enabling researchers worldwide to automatically analyse brain MRIs at a higher level of granularity, NextBrain holds promise to increase the specificity of findings and accelerate our quest to understand the human brain in health and disease.},
  year = {2025},
  journal = {Nature},
  pages = {1-8},
  month = {2025-11-05},
  issn = {1476-4687},
  url = {https://www.nature.com/articles/s41586-025-09708-2},
  doi = {10.1038/s41586-025-09708-2},
  language = {en},
}