@article{6086,
  keywords = {brain microvascular networks, endothelial dysfunction, flavivirus non-structural protein 1, microfluidic chips},
  author = {Monika Rajput and Yen-Ting Tung and Yu-Chi Chen and Min Jae Song and Marc Ferrer and Emily M. Lee},
  title = {Perfusable Brain Microvascular Network-On-Chip Model to Study Flavivirus NS1-Induced Endothelial Dysfunction},
  abstract = {Flaviviruses can lead to severe neurotropic-encephalitic pathology, for which the viral nonstructural protein 1 (NS1) plays a major role. 2D in vitro cellular systems and animal models are currently used to investigate the effects of flavivirus NS1 in the brain-blood barrier do not fully capture pathogenesis and drug responses seen in humans. Here is reported the development of a perfusable human brain microvascular network-on-a-chip (BMVasChip) model which incorporates brain endothelial cells and pericytes and recapitulates the cellular organization and expression of brain blood barrier-specific markers, including intracellular junction proteins. This BMVasChip model is used to investigate vasculopathy caused by exposure to a panel of flavivirus NS1 proteins, including Japanese encephalitis (JEV), West Nile (WNV), and dengue virus (DENV) NS1 proteins. The BMVasChip platform is able to demonstrate flavivirus NS1-induced pathophysiological phenotypes in the BMVasChip, including early and late endothelial activation and dysfunction features such as loss of barrier integrity, morphological features, and production of pro-inflammatory markers in a virus, dose, and time-dependent manner. In summary, the BMVasChip model recapitulates key features of endothelial dysfunction, potentially reflecting NS1's role in viral pathogenesis and providing targets for antiviral therapeutics and vaccine development.},
  journal = {Advanced Functional Materials},
  volume = {n/a},
  pages = {e04476},
  issn = {1616-3028},
  url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.202504476},
  doi = {10.1002/adfm.202504476},
  language = {en},
}