@article{5611,
  keywords = {Cancer, cell culture, Cell-based assays},
  author = {Stina Oredsson and Atena Malakpour-Permlid and Johan Zhu and Tilo Weber},
  title = {Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures},
  abstract = {The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures. Here, we present a protocol for the use of OUR medium for routine culturing, cell banking, and medium modification for suspension culture. We describe steps for thawing, sub-culturing, and freezing cells. We then detail procedures for using these techniques for VERO, A549, THP-1, and Jurkat cells.},
  year = {2025},
  journal = {STAR Protocols},
  volume = {6},
  pages = {103781},
  month = {2025-06-20},
  issn = {2666-1667},
  url = {https://www.sciencedirect.com/science/article/pii/S266616672500187X},
  doi = {10.1016/j.xpro.2025.103781},
}