@article{5606,
  keywords = {Animals, Bioprinting, Cell Survival, Collagen, extracellular matrix, Humans, Hydrogels, Printing, Three-Dimensional, Tissue engineering, Tissue Scaffolds},
  author = {Daniel J. Shiwarski and Andrew R. Hudson and Joshua W. Tashman and Ezgi Bakirci and Samuel Moss and Brian D. Coffin and Adam W. Feinberg},
  title = {3D bioprinting of collagen-based high-resolution internally perfusable scaffolds for engineering fully biologic tissue systems},
  abstract = {Organ-on-a-chip and microfluidic systems have improved the translational relevance of in vitro systems; however, current manufacturing approaches impart limitations on materials selection, non-native mechanical properties, geometric complexity, and cell-driven remodeling into functional tissues. Here, we three-dimensionally (3D) bioprint extracellular matrix (ECM) and cells into collagen-based high-resolution internally perfusable scaffolds (CHIPS) that integrate with a vascular and perfusion organ-on-a-chip reactor (VAPOR) to form a complete tissue engineering platform. We improve the fidelity of freeform reversible embedding of suspended hydrogels (FRESH) bioprinting to produce a range of CHIPS designs fabricated in a one-step process. CHIPS exhibit size-dependent permeability of perfused molecules into the surrounding scaffold to support cell viability and migration. Lastly, we implemented multi-material bioprinting to control 3D spatial patterning, ECM composition, cellularization, and material properties to create a glucose-responsive, insulin-secreting pancreatic-like CHIPS with vascular endothelial cadherin+ vascular-like networks. Together, CHIPS and VAPOR form a platform technology toward engineering full organ-scale function for disease modeling and cell replacement therapy.},
  year = {2025},
  journal = {Science Advances},
  volume = {11},
  pages = {eadu5905},
  month = {2025-04-25},
  issn = {2375-2548},
  doi = {10.1126/sciadv.adu5905},
  language = {eng},
}