@article{bibcite_5526,
  keywords = {biomaterials, Biomedical Engineering, Engineering, Pharmaceutics},
  author = {Rodi Kado Abdalkader and Satoshi Konishi and Takuya Fujita},
  title = {Development of a flexible 3D printed TPU-PVC microfluidic devices for organ-on-a-chip applications},
  abstract = {The development of cost-effective, flexible, and scalable microfluidic devices is crucial for advancing organ-on-a-chip (OoC) technology for drug discovery and disease modeling applications. In this study, we present a novel 3D-printed flexible microfluidic device (3D-FlexTPU-MFD) fabricated through a one-step fused deposition modeling (FDM) process using thermoplastic polyurethane (TPU) as the printing filament and polyvinyl chloride (PVC) as the bonding substrate. The device{\textquoteright}s compatibility was evaluated with various cell types, including human primary myoblasts, human primary endothelial cells (HUVEC), and human iPSC-derived optic vesicle (OV) organoids. Myoblasts cultured within the device exhibited high viability, successful differentiation, and the formation of aligned myotube bundles, outperforming conventional well-plate cultures. Additionally, iPSC-derived OV organoids-maintained viability, displayed neurite outgrowth, and sustained expression of the eye marker PAX6. These results demonstrate that the 3D-FlexTPU-MFD effectively supports cell growth, differentiation, and alignment, making it a promising platform for tissue modeling and OoC applications in future.},
  year = {2025},
  journal = {Scientific Reports},
  volume = {15},
  pages = {6125},
  month = {2025-02-19},
  issn = {2045-2322},
  url = {https://www.nature.com/articles/s41598-025-90470-w},
  doi = {10.1038/s41598-025-90470-w},
  language = {en},
}