@article{bibcite_5451, keywords = {Biotechnology and bioengineering, Chemistry, Material sciences, Tissue engineering}, author = {Tamilselvan Mohan and Matej Bra{\v c}i{\v c} and Doris Bra{\v c}i{\v c} and Florian Lackner and Chandran Nagaraj and Andreja Dobaj {\v S}tiglic and Rupert Kargl and Karin Stana Kleinschek}, title = {Protocol for the fabrication of self-standing (nano)cellulose-based 3D scaffolds for tissue engineering}, abstract = {Three-dimensional (3D) and porous scaffolds made from nanocellulosic materials hold significant potential in tissue engineering (TE). Here, we present a protocol for fabricating self-standing (nano)cellulose-based 3D scaffolds designed for in~vitro testing of cells from skin and cartilage tissues. We describe steps for preparation of nanocellulose ink, scaffold formation using 3D printing, and freeze-drying.~We then detail post-processing procedures to enhance mechanical properties, stability, and biocompatibility. This protocol offers researchers a framework for developing versatile and sustainable biomaterials for regenerative medicine. For complete details on the use and execution of this protocol, please refer to Mohan et~al.1 and {\v S}tiglic et~al.2}, year = {2025}, journal = {STAR Protocols}, volume = {6}, pages = {103583}, month = {2025-03-21}, issn = {2666-1667}, url = {https://www.sciencedirect.com/science/article/pii/S2666166724007482}, doi = {10.1016/j.xpro.2024.103583}, }