@article{5446,
  author = {Dae-Seop Shin and Myung Jin Son and Myungae Bae and Hyunwoo Kim},
  title = {Local Stiffness Measurement of Hepatic Steatosis Model Liver Organoid by Fluorescence Imaging-Assisted Probe Indentation},
  abstract = {Mechanical stiffness of liver organoid is a key indicator for the progress of hepatic steatosis. Probe indentation is a noninvasive methodology to measure Young’s modulus (YM); however, the inhomogeneous nature of the liver organoid induces measurement uncertainty requiring a large number of indentations covering a wide scanning area. Here, we demonstrate that lipid-stained fluorescence imaging-assisted probe indentation significantly reduces the number of measurements by specifying the highly lipid-induced area. Lipid-stained hepatic steatosis model liver organoid shows broad fluorescence distributions that are spatially correlated with a decreased YM on a lipid-filled region with bright fluorescence compared with that measured on a blank region with dark fluorescence. The organoid viability remained robust even after exposure to an ambient condition up to 6 h, showing that probe indentations can be noninvasive methods for liver organoid stiffness measurements.},
  year = {2024},
  journal = {ACS Biomaterials Science & Engineering},
  volume = {10},
  pages = {7386-7393},
  month = {2024-12-09},
  url = {https://doi.org/10.1021/acsbiomaterials.4c01242},
  doi = {10.1021/acsbiomaterials.4c01242},
}